def __eq__(self, other):
if not IsoRequestPosition.__eq__(self, other): return False
if not self.is_empty:
if not self.reagent_name == other.reagent_name:
return False
if not self.reagent_dil_factor == other.reagent_dil_factor:
return False
if not (self.is_mock or self.is_empty):
if not self.final_concentration == other.final_concentration:
return False
return True
def __eq__(self, other):
if not IsoRequestPosition.__eq__(self, other): return False
if not self.is_empty:
if not self.reagent_name == other.reagent_name:
return False
if not self.reagent_dil_factor == other.reagent_dil_factor:
return False
if not (self.is_mock or self.is_empty):
if not self.final_concentration == other.final_concentration:
return False
return True
def __init__(self,
rack_position,
molecule_design_pool=None,
position_type=None,
reagent_name=None,
reagent_dil_factor=None,
iso_volume=None,
iso_concentration=None,
final_concentration=None,
optimem_dil_factor=None):
"""
:param rack_position: The rack position.
:type rack_position: :class:`thelma.entities.rack.RackPosition`.
:param molecule_design_pool: The molecule design pool or placeholder for
the RNAi reagent.
:type molecule_design_pool: :class`int` (ID), :class:`str` (placeholder)
or :class:`thelma.entities.moleculedesign.StockSampleMoleculeDesign`
:param position_type: influences valid values for other parameters
:type position_type: :class:`str
:param reagent_name: The name of the transfection reagent.
:type reagent_name: :class:`str`
:param reagent_dil_factor: The final dilution factor of the
transfection reagent in the cell plate.
:type reagent_dil_factor: positive number, no unit
:param iso_volume: The volume requested by the stock management.
:type iso_volume: positive number, unit ul
:param iso_concentration: The concentration requested by the stock
management.
:type iso_concentration: positive number, unit nM
:param final_concentration: The final concentration of the RNAi
reagent in the cell plate.
:type final_concentration: positive number, unit nM
:param optimem_dil_factor: The dilution factor for the OptiMem dilution
(use only if you do not want to use the default factor).
:type optimem_dil_factor: positive number
"""
IsoRequestPosition.__init__(self,
rack_position=rack_position,
molecule_design_pool=molecule_design_pool,
position_type=position_type,
iso_volume=iso_volume,
iso_concentration=iso_concentration)
#: Stores the position in the cell plate that are filled by this
#: source positions (:class:`set` of
#: :class:`thelma.entities.rack.RackPosition` objects).
self.cell_plate_positions = set()
#: The name of the RNAi reagent.
self.reagent_name = reagent_name
#: The final dilution factor RNAi reagent in the cell plate.
self.reagent_dil_factor = get_converted_number(reagent_dil_factor)
#: The final concentration in the cell plate (experiment plate).
self.final_concentration = get_converted_number(final_concentration)
#: The optimem dilution factor set in library screenings (because
#: in this case it is depending on the final concentration instead
#: of depending on the molecule type).
self._optimem_dil_factor = optimem_dil_factor
tf_attrs = [('reagent name', self.reagent_name),
('reagent dilution factor', self.reagent_dil_factor),
('final concentration', self.final_concentration),
('optimem dilution factor', self._optimem_dil_factor)]
if self.is_untreated_type:
self._check_untreated_values(tf_attrs)
elif self.is_empty:
self._check_none_value(tf_attrs)
else:
if self.reagent_name is not None and \
(not isinstance(self.reagent_name, basestring) or \
len(self.reagent_name) < 2):
msg = 'The reagent name must be at least 2 characters long ' \
'if there is one (obtained: "%s")!' % (self.reagent_name)
raise ValueError(msg)
numericals = [tf_attrs[1], tf_attrs[3]]
if self.is_mock:
self._check_mock_values([tf_attrs[2]])
else:
numericals.append(tf_attrs[2])
self._check_numbers(numericals, allow_none=True)
def __init__(self, rack_position, molecule_design_pool=None,
position_type=None, reagent_name=None, reagent_dil_factor=None,
iso_volume=None, iso_concentration=None,
final_concentration=None, optimem_dil_factor=None):
"""
:param rack_position: The rack position.
:type rack_position: :class:`thelma.entities.rack.RackPosition`.
:param molecule_design_pool: The molecule design pool or placeholder for
the RNAi reagent.
:type molecule_design_pool: :class`int` (ID), :class:`str` (placeholder)
or :class:`thelma.entities.moleculedesign.StockSampleMoleculeDesign`
:param position_type: influences valid values for other parameters
:type position_type: :class:`str
:param reagent_name: The name of the transfection reagent.
:type reagent_name: :class:`str`
:param reagent_dil_factor: The final dilution factor of the
transfection reagent in the cell plate.
:type reagent_dil_factor: positive number, no unit
:param iso_volume: The volume requested by the stock management.
:type iso_volume: positive number, unit ul
:param iso_concentration: The concentration requested by the stock
management.
:type iso_concentration: positive number, unit nM
:param final_concentration: The final concentration of the RNAi
reagent in the cell plate.
:type final_concentration: positive number, unit nM
:param optimem_dil_factor: The dilution factor for the OptiMem dilution
(use only if you do not want to use the default factor).
:type optimem_dil_factor: positive number
"""
IsoRequestPosition.__init__(self, rack_position=rack_position,
molecule_design_pool=molecule_design_pool,
position_type=position_type, iso_volume=iso_volume,
iso_concentration=iso_concentration)
#: Stores the position in the cell plate that are filled by this
#: source positions (:class:`set` of
#: :class:`thelma.entities.rack.RackPosition` objects).
self.cell_plate_positions = set()
#: The name of the RNAi reagent.
self.reagent_name = reagent_name
#: The final dilution factor RNAi reagent in the cell plate.
self.reagent_dil_factor = get_converted_number(reagent_dil_factor)
#: The final concentration in the cell plate (experiment plate).
self.final_concentration = get_converted_number(final_concentration)
#: The optimem dilution factor set in library screenings (because
#: in this case it is depending on the final concentration instead
#: of depending on the molecule type).
self._optimem_dil_factor = optimem_dil_factor
tf_attrs = [('reagent name', self.reagent_name),
('reagent dilution factor', self.reagent_dil_factor),
('final concentration', self.final_concentration),
('optimem dilution factor', self._optimem_dil_factor)]
if self.is_untreated_type:
self._check_untreated_values(tf_attrs)
elif self.is_empty:
self._check_none_value(tf_attrs)
else:
if self.reagent_name is not None and \
(not isinstance(self.reagent_name, basestring) or \
len(self.reagent_name) < 2):
msg = 'The reagent name must be at least 2 characters long ' \
'if there is one (obtained: "%s")!' % (self.reagent_name)
raise ValueError(msg)
numericals = [tf_attrs[1], tf_attrs[3]]
if self.is_mock:
self._check_mock_values([tf_attrs[2]])
else:
numericals.append(tf_attrs[2])
self._check_numbers(numericals, allow_none=True)
