# * DISPENSE WATER ------------------------------------------
# Program Variables
transfer_volume = 180
blowoff_volume = 0
soloSoft.getTip()
for i in range(1, 3): # columns in 12 channel reservoir
for j in range(1, 7): # <- columns in corning 3635 clearUV
soloSoft.aspirate(
position="Position3",
aspirate_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup().setColumn(
i, transfer_volume
),
aspirate_shift=[
0,
0,
4,
], # larger shift needed for 12 channel resevoir <- check this with new plate entries
pre_aspirate=blowoff_volume,
)
soloSoft.dispense(
position="Position6",
dispense_volumes=Plate_96_Corning_3635_ClearUVAssay().setColumn(
(((i - 1) * 6) + j), transfer_volume
),
dispense_shift=[0, 0, 2],
blowoff=blowoff_volume,
)
# DISPENSE BUFFER ------------------------------------------
soloSoft = SoloSoft(
filename="cherry_picking_test.hso",
plateList=[
"TipBox.50uL.Axygen-EV-50-R-S.tealbox",
"Empty",
"Empty",
"Empty",
"Empty",
"Empty",
"Empty",
"Plate.96.Corning-3635.ClearUVAssay",
],
)
soloSoft.getTip()
soloSoft.aspirate(
position="Position8",
aspirate_volumes=Plate_96_Corning_3635_ClearUVAssay().setColumn(1, 50),
aspirate_shift=[0, 0, 2],
)
for i in range(3):
soloSoft.dispense(
position="Position8",
dispense_volumes=Plate_96_Corning_3635_ClearUVAssay().setColumn(2, 10),
dispense_shift=[0, 0, 2],
)
soloSoft.shuckTip()
soloSoft.savePipeline()
"TipBox.50uL.Axygen-EV-50-R-S.tealbox",
"Empty",
],
)
# * STEP 1: Transfer Cells ------------------------------------------------------------
soloSoft.getTip(
) # 200uL tips -> all transfers are same cells, OK to keep same tips for all of step 1
for i in range(1, 3):
for j in range(1, 7):
soloSoft.aspirate(
position="Position3",
aspirate_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup(
).setColumn(i, cell_transfer_volume),
aspirate_shift=[0, 0, cell_aspirate_z_shift],
pre_aspirate=cell_blowoff,
mix_at_start=
True, # mix cells before aspirating them -> probably a good idea
mix_volume=cell_mix_volume,
mix_cycles=cell_num_mixes,
dispense_height=cell_aspirate_z_shift,
)
soloSoft.dispense(
position="Position4",
dispense_volumes=Plate_96_Corning_3635_ClearUVAssay().setColumn(
(6 * (i - 1)) + j, cell_transfer_volume),
dispense_shift=[0, 0, default_z_shift],
blowoff=cell_blowoff,
# no need to mix because it will shake in the Hidex
)
# for testing
"Plate.96.PlateOne-1833-9600.ConicalBottomStorage",
"Plate.96.PlateOne-1833-9600.ConicalBottomStorage",
"TipBox.50uL.Axygen-EV-50-R-S.tealbox",
"Empty",
],
)
soloSoft.getTip()
for i in range(12):
soloSoft.aspirate(
position="Position3",
aspirate_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup().
setColumn(aspirate_column, aspirate_volume),
aspirate_shift=[0, 0, default_z_shift],
pre_aspirate=default_blowoff,
# mix_at_start=True,
# mix_cycles=aspirate_num_mixes,
# mix_volume=aspirate_mix_volume,
# dispense_height=default_z_shift,
syringe_speed=aspirate_syringe_speed,
)
soloSoft.dispense(
position="Position3",
dispense_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup().
setColumn(dispense_column, aspirate_volume),
dispense_shift=[0, 0, default_z_shift],
blowoff=default_blowoff,
syringe_speed=aspirate_syringe_speed,
)
soloSoft.shuckTip()
"Reservoir.12col.Agilent-201256-100.BATSgroup",
"Empty",
"Plate.96.Corning-3635.ClearUVAssay",
"Plate.96.Corning-3635.ClearUVAssay",
"TipBox.50uL.Axygen-EV-50-R-S.tealbox",
"AgarPlate.40mL.OmniTray-242811.ColonyPicker",
],
)
# * STEP 1: Dispense diluent into whole plate -> MUST CHECK/REFILL BETWEEN CREATING EACH PLATE -------------
soloSoft.getTip()
# Don't dispense diluent into first column of each half plate -> will be pure stock
for i in range(2, 7): # first half of the plate, columns 2-6
soloSoft.aspirate(
position="Position3",
aspirate_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup().
setColumn(media_aspirate_column, dilution_media_volume),
aspirate_shift=[0, 0, 4],
)
soloSoft.dispense(
position="Position6",
dispense_volumes=Plate_96_Corning_3635_ClearUVAssay().setColumn(
i, dilution_media_volume),
dispense_shift=[0, 0, default_z_shift],
)
media_aspirate_column += 1
for i in range(8, 13): # second half of the plate, columns 8-12
soloSoft.aspirate(
position="Position3",
aspirate_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup().
setColumn(media_aspirate_column, dilution_media_volume),
aspirate_shift=[0, 0, 4],
"Plate.96.Corning-3635.ClearUVAssay",
"Plate.96.Corning-3635.ClearUVAssay",
"Empty",
],
)
# * Fill 6 columns of empty 96 well plate (corning 3383 or Falcon - ref 353916) with fresh lb media (12 channel in Position 3, column 1)
soloSoft.getTip()
j = 1
for i in range(1, 7):
soloSoft.aspirate(
position="Position3",
aspirate_volumes=Reservoir_12col_Agilent_201256_100_BATSgroup().setColumn(
1, media_transfer_volume_s1
),
aspirate_shift=[
0,
0,
4,
], # larger shift needed for 12 channel reservoir #TODO remeasure 12 channel reservoir depth
)
soloSoft.dispense(
position="Position4",
dispense_volumes=Plate_96_Corning_3635_ClearUVAssay().setColumn(
i, media_transfer_volume_s1
),
dispense_shift=[0, 0, 2],
)
# * Fill first column of culture 10 fold dilution plate with fresh lb media
soloSoft.aspirate(
