def phase_stretch_transform(img, LPF, S, W, threshold_min, threshold_max, flag):
L = 0.5
x = np.linspace(-L, L, img.shape[0])
y = np.linspace(-L, L, img.shape[1])
[X1, Y1] = (np.meshgrid(x, y))
X = X1.T
Y = Y1.T
theta, rho = cart2pol(X, Y)
orig = ((np.fft.fft2(img)))
expo = np.fft.fftshift(np.exp(-np.power((np.divide(rho, math.sqrt((LPF ** 2) / np.log(2)))), 2)))
orig_filtered = np.real(np.fft.ifft2((np.multiply(orig, expo))))
PST_Kernel_1 = np.multiply(np.dot(rho, W), np.arctan(np.dot(rho, W))) - 0.5 * np.log(
1 + np.power(np.dot(rho, W), 2))
PST_Kernel = PST_Kernel_1 / np.max(PST_Kernel_1) * S
temp = np.multiply(np.fft.fftshift(np.exp(-1j * PST_Kernel)), np.fft.fft2(orig_filtered))
orig_filtered_PST = np.fft.ifft2(temp)
PHI_features = np.angle(orig_filtered_PST)
if flag == 0:
out = PHI_features
else:
features = np.zeros((PHI_features.shape[0], PHI_features.shape[1]))
features[PHI_features > threshold_max] = 1
features[PHI_features < threshold_min] = 1
features[img < (np.amax(img) / 20)] = 0
out = features
out = mh.thin(out, 1)
out = mh.bwperim(out, 4)
out = mh.thin(out, 1)
out = mh.erode(out, np.ones((1, 1)))
return out, PST_Kernel
def pst_algorithm(image, LPF, Phase_strength, Warp_strength, Threshold_min,
Threshold_max, Morph_flag):
L = 0.5
x = np.linspace(-L, L, image.shape[0])
y = np.linspace(-L, L, image.shape[1])
[X1, Y1] = (np.meshgrid(x, y))
X = X1.T
Y = Y1.T
[THETA, RHO] = [np.arctan2(Y, X),
np.hypot(X, Y)] # cartesian to polar coordinates
# Apply localization kernel to the original image to reduce noise
Image_orig_f = np.fft.fft2(image)
expo = np.fft.fftshift(
np.exp(-np.power((np.divide(RHO, math.sqrt((LPF**2) /
np.log(2)))), 2)))
Image_orig_filtered = np.real(
np.fft.ifft2((np.multiply(Image_orig_f, expo))))
# Constructing the PST Kernel
PST_Kernel_1 = np.multiply(
np.dot(RHO, Warp_strength), np.arctan(np.dot(RHO, Warp_strength))
) - 0.5 * np.log(1 + np.power(np.dot(RHO, Warp_strength), 2))
PST_Kernel = PST_Kernel_1 / np.max(PST_Kernel_1) * Phase_strength
# Apply the PST Kernel
temp = np.multiply(np.fft.fftshift(np.exp(-1j * PST_Kernel)),
np.fft.fft2(Image_orig_filtered))
Image_orig_filtered_PST = np.fft.ifft2(temp)
# Calculate phase of the transformed image
PHI_features = np.angle(Image_orig_filtered_PST)
if Morph_flag == 0:
out = PHI_features
out = (out / np.max(out)) * 3
else:
# find image sharp transitions by thresholding the phase
features = np.zeros((PHI_features.shape[0], PHI_features.shape[1]))
features[PHI_features > Threshold_max] = 1 # Bi-threshold decision
features[
PHI_features <
Threshold_min] = 1 # as the output phase has both positive and negative values
features[image < (
np.amax(image) / 20
)] = 0 # Removing edges in the very dark areas of the image (noise)
# apply binary morphological operations to clean the transformed image
out = features
out = mh.thin(out, 1)
out = mh.bwperim(out, 4)
out = mh.thin(out, 1)
out = mh.erode(out, np.ones((1, 1)))
return out
def phase_stretch_transform(img, LPF, S, W, Threshold_min, Threshold_max,
flag):
L = 0.5
x = np.linspace(-L, L, img.shape[0])
y = np.linspace(-L, L, img.shape[1])
X, Y = np.meshgrid(x, y)
p, q = X.T, y.T
theta, rho = cart2pol(p, q)
# 接下来对PST公式从右至左依次实现，
# 对输入图像进行快速傅里叶变换,
orig = np.fft.fft2(img)
# 实现L[p, q]
expo = np.fft.fftshift(
np.exp(-np.power((np.divide(rho, math.sqrt((LPF**2) /
np.log(2)))), 2)))
# 对图像进行平滑处理，
orig_filtered = np.real(np.fft.ifft2((np.multiply(orig, expo))))
# 实现相位核，
PST_Kernel_1 = np.multiply(np.dot(rho, W), np.arctan(np.dot(
rho, W))) - 0.5 * np.log(1 + np.power(np.dot(rho, W), 2))
PST_Kernel = PST_Kernel_1 / np.max(PST_Kernel_1) * S
# 将前面实现的部分与相位核做乘积，
temp = np.multiply(np.fft.fftshift(np.exp(-1j * PST_Kernel)),
np.fft.fft2(orig_filtered))
# 对图像进行逆快速傅里叶变换，
orig_filtered_PST = np.fft.ifft2(temp)
# 进行角运算，得到变换图像的相位，
PHI_features = np.angle(orig_filtered_PST)
if flag == 0:
out = PHI_features
else:
# 对图像进行阈值化处理，
features = np.zeros((PHI_features.shape[0], PHI_features.shape[1]))
features[PHI_features > Threshold_max] = 1
features[PHI_features < Threshold_min] = 1
features[img < (np.amax(img) / 20)] = 0
# 应用二进制形态学操作来清除转换后的图像,
out = features
out = mh.thin(out, 1)
out = mh.bwperim(out, 4)
out = mh.thin(out, 1)
out = mh.erode(out, np.ones((1, 1)))
return out, PST_Kernel
def count_holes(dir_name, extension='.tif', plotting = False, dpi = 300, transparent=True):
total_holes = []
i = 0
for files in sorted(os.listdir(dir_name)):
if files.endswith(extension):
scan = os.path.join(dir_name,files)
img = tf.imread(scan)
img[img > 0] = -1
skel = mh.thin(img)
noholes = mh.morph.close_holes(skel)
cskel = np.logical_not(skel)
choles = np.logical_not(noholes)
holes = np.logical_and(cskel,noholes)
lab, n = mh.label(holes)
total_holes.append(n)
if plotting:
fig = plt.figure()
plt.imshow(img)
plt.tight_layout(pad=0)
fig.savefig(dir_name + '/binary_{}_{:03d}.png'.format(dir_name,i+1), dpi=dpi, transparent=transparent)
plt.close(fig)
fig = plt.figure()
plt.imshow(skel)
plt.tight_layout(pad=0)
fig.savefig(dir_name + '/skel_{}_{:03d}.png'.format(dir_name,i+1), dpi=dpi, transparent=transparent)
plt.close(fig)
fig = plt.figure()
plt.imshow(lab)
plt.tight_layout(pad=0)
fig.savefig(dir_name + '/labels_{}_{:03d}.png'.format(dir_name,i+1), dpi=dpi, transparent=transparent)
plt.close(fig)
i += 1
return total_holes
def skeleton(self):
self.image = removeframe(readgreyimage(self.image))
self.ip = dealwhitelabel(self.image, what='remove', ontooriginal=False)
# self.ip=openclose(self.ip,open=False)
self.sk = mh.thin(self.ip > 127)
self.sk = pruning(self.sk, size=15)
return self
def chromatids_elements(TopHatedChromosome):
'''Take a High pass filtered (or top hat) image of a chromosome and label the chromatids elements
'''
threshed = TopHatedChromosome > 0
#threshed = mh.open(threshed)
labthres, _ = mh.label(threshed)
labsz = mh.labeled.labeled_size(labthres)
mh.labeled.remove_regions_where(labthres, labsz < 2, inplace=True)
threshed = labthres > 0
skel2 = mh.thin(threshed)
bp2 = branchedPoints(skel2, showSE=False) > 0
rem = np.logical_and(skel2, np.logical_not(bp2))
labskel, _ = mh.labeled.label(rem)
#print labskel.dtype
size_sk = mh.labeled.labeled_size(labskel)
#print size_sk
skelem = mh.labeled.remove_regions_where(labskel, size_sk < 4)
distances = mh.stretch(mh.distance(threshed))
surface = (distances.max() - distances)
chr_label = mh.cwatershed(surface, skelem)
#print chr_label.dtype, type(chr_label)
chr_label *= threshed
#This convertion is important !!
chr_label = chr_label.astype(np.intc)
#-------------------------------
mh.labeled.relabel(chr_label, inplace=True)
labsize2 = mh.labeled.labeled_size(chr_label)
cleaned = mh.labeled.remove_regions_where(chr_label, labsize2 < 8)
mh.labeled.relabel(cleaned, inplace=True)
return cleaned
def objectfeatures(img):
"""
values=objectfeatures(img)
This implements the object features described in
"Object Type Recognition for Automated Analysis of Protein Subcellular Location"
by Ting Zhao, Meel Velliste, Michael V. Boland, and Robert F. Murphy
in IEEE Transaction on Image Processing
"""
protimg = img.get("procprotein")
dnaimg = img.channeldata.get("procdna", None)
assert (
dnaimg is None or protimg.shape == dnaimg.shape
), "pymorph.objectfeatures: DNA image is not of same size as Protein image."
labeled, N = ndimage.label(protimg, ones((3, 3)))
if not N:
return np.zeros((0, 11))
sofs = np.zeros((N, 11))
indices = np.arange(1, N + 1)
if dnaimg is not None:
dnacofy, dnacofx = ndimage.center_of_mass(dnaimg)
bindna = dnaimg > 0
# According to the documentation, it shouldn't matter if indices is None,
# but in my version of scipy.ndimage, you *have* to use indices.
centers = ndimage.center_of_mass(protimg, labeled, indices)
if N == 1:
centers = list(centers)
centers = np.asarray(centers)
centers -= np.array((dnacofy, dnacofx))
centers **= 2
sofs[:, 1] = np.sqrt(centers.sum(1))
locations = ndimage.find_objects(labeled, N)
sofs[:, 9] = ndimage.measurements.sum(protimg, labeled, indices)
for obji in xrange(N):
slice = locations[obji]
binobj = (labeled[slice] == (obji + 1)).copy()
protobj = protimg[slice]
binskel = thin(binobj)
objhull = convexhull(binobj)
no_of_branch_points = fast_sum(find_branch_points(binskel))
hfeats = hullfeatures(binobj, objhull)
sofs[obji, 0] = fast_sum(binobj)
if dnaimg is not None:
sofs[obji, 2] = fast_sum(binobj & bindna[slice])
sofs[obji, 3] = hfeats[2]
sofs[obji, 4] = euler(binobj)
sofs[obji, 5] = hfeats[1]
sofs[obji, 6] = fast_sum(binskel)
sofs[obji, 7] = hfeats[0]
sofs[obji, 9] /= fast_sum(binskel * protobj)
sofs[obji, 10] = no_of_branch_points
sofs[:, 2] /= sofs[:, 0]
sofs[:, 8] = sofs[:, 6] / sofs[:, 0]
sofs[:, 10] /= sofs[:, 6]
return sofs
def skel(self, img):
img[0,:]=0 # make 1st line in the image black to achieve consistent result between distance field and medial axis skeleton.
img[len(img)-1,:]=0 # make last line in the image black to achieve consistent result between distance field and medial axis skeleton.
img[:,len(img[0])-1]=0 # make right column in the image black to achieve consistent result between distance field and medial axis skeleton.
img[:,0]=0 # make left column in the image black to achieve consistent result between distance field and medial axis skeleton.
dmap = m.distance(img>0,metric='euclidean')
dmap=np.sqrt(dmap)*2
skelImg=m.thin(img>0)
return skelImg, dmap
def _objskelfeats(objimg):
"""
feats = _objskelfeats(objimg)
Calculate skeleton features for the object OBJIMG.
"""
objimg = objimg
objbin = objimg > 0
objsize = objbin.sum()
if objsize == 0:
return numpy.zeros(5)
objskel = thin(objbin)
skellen = objskel.sum()
skelhull = convexhull(objskel);
hullsize = skelhull.sum()
hullsize = max(hullsize, skellen) # Corner cases such as [[1]]
skel_hull_area_ratio = skellen / hullsize
skel_obj_area_ratio = skellen/objsize
skel_fluor = (objimg * objskel).sum()
obj_fluor = objimg.sum()
skel_obj_fluor_ratio = skel_fluor/obj_fluor
branch_points = find_branch_points(objskel)
no_of_branch_points = branch_points.sum()
return numpy.array([
skellen,
skel_hull_area_ratio,
skel_obj_area_ratio,
skel_obj_fluor_ratio,
no_of_branch_points/skellen])
import numpy as np
import mahotas
from pylab import imshow, savefig
A = np.zeros((100,100), bool)
A[40:60] = 1
W = mahotas.thin(A)
D = mahotas.distance(~W)
imshow(D)
savefig('distance.png')
def n_skeleton_branched_points(image):
"""Number of branched points of skeleton."""
skeleton = mh.thin(image)
b_points = branched_points(skeleton)
return (sum(sum(b_points != False)))
def processImage(filepath, median_filter_size=15, small_object_size=40, fill_small_holes_n_iterations=2, n_prune=15,
bg_greyscale=250, crack_greyscale=245):
"""
Function to create extract a binary image pixel-thick cracks from an image of a cracked material.
For details, see Griffiths et al. (2017) and supplementary materials (provided in ./references folder).
Griffiths, L., Heap, M.J., Baud, P., Schmittbuhl, J., 2017. Quantification of microcrack characteristics and
implications for stiffness and strength of granite. International Journal of Rock Mechanics and Mining Sciences 100,
138–150. https://doi.org/10.1016/j.ijrmms.2017.10.013
Args:
filepath (string): path to the image file to be processed
median_filter_size (int): width (in pixels) of the square median filter window. the value at the center of the
moving window is replaces by the median of the values within the window. This should be set to a value just
larger than the crack width.
small_object_size (int): connected objects within the image following thresholding, with contained within a
square of this size (in pixels) will be removed from the image.
fill_small_holes_n_iterations (int): Before skeletonisation of the segmented image, any holes in the segmented
cracks must be removed. This value gives the number of times the image will be dilated and eroded, to fill all
holes. 2 is often enough.
n_prune: Number of times the skeletonised cracks are pruned i.e., end points are removed. This value should be
greater than the width of the segmented cracks before skeletonisation, or the median_filter_size, which should
be similar.
bg_greyscale: The lower boundary of the image mask defined prior to the watershed segmentation. Any pixel with
a greyscale value below this value is "background"
crack_greyscale: The lower boundary of the image mask defined prior to the watershed segmentation. Any pixel
with a greyscale value above this value is "crack".
Returns:
cracks_skeleton_restored (numpy array): Binary image of the skeletonised cracks.
"""
# img_orig = np.uint8( scipy.misc.imread(filename, flatten = True) )
image = Image.open(filepath).convert('L')
img_orig = np.uint8(image)
# img = img[2:-2, 2:-2] # Microscope image borders are all same grayscale
# img = img[760:780, 923:977]
""" Filter image """
img = (255 - img_orig) # Switch grayscale
img_median = ndi.median_filter(img, median_filter_size) # Median filter, good at conserving edges
img_filtered = (255 - cv2.subtract(img, img_median)) # Subtract filtered image from original (cracks = white)
""" Segmentation """
markers = np.zeros_like(img_filtered) # Mark the different regions of the image
markers[img_filtered > bg_greyscale] = 1 # Minimum crack grayscales
markers[img_filtered < crack_greyscale] = 2 # Maximum crack grayscales
# Plot median filtered image and mask
f, ax = plt.subplots(1, 2, sharex=True, sharey=True)
ax[0].imshow(img_filtered, cmap='gray')
ax[1].imshow(markers, cmap='gray')
plt.show()
elevation_map = filters.sobel(img_filtered) # Edge detection for watershed
segmented = np.abs(255 - 255 * watershed(elevation_map, markers)) # Watershed segmentation, white on black
""" Thin, prune and label cracks """
cracks = removeSmallObjects(segmented, small_object_size) # Remove small objects
cracks = fillSmallHoles(cracks, fill_small_holes_n_iterations) # Fill small holes in cracks
cracks = binary_dilation(cracks) # Dilate before thinning
cracks_skeleton = 255 * np.int8(mh.thin(cracks > 0)) # Skeletonise image
cracks_skeleton_pruned = removeEndPoints(cracks_skeleton, n_prune) # Remove skeletonisation artefacts
cracks_skeleton_pruned_no_bp = removeBranchPoints(cracks_skeleton_pruned) # Remove branch points to separate cracks
cracks_skeleton_pruned_no_bp_2_ep = removeEndPointsIter(
cracks_skeleton_pruned_no_bp) # Remove end points until 2 per crack
cracks_skeleton_restored = restoreBranches(cracks_skeleton_pruned_no_bp_2_ep,
cracks_skeleton) # Restore branches without creating new endpoints
# Plot original and final image
f, ax = plt.subplots(1, 2, sharex=True, sharey=True)
ax[0].imshow(img_orig, cmap='gray')
ax[1].imshow(cracks_skeleton_restored, cmap='gray')
plt.show()
# Save images
save_image.counter = 0
save_image(img_orig)
save_image(255 - img_median)
save_image(255 - img_filtered)
save_image(255 * elevation_map / np.max(elevation_map))
save_image(255 * segmented / np.max(segmented))
save_image(255 * cracks / np.max(cracks))
save_image(255 * cracks_skeleton / np.max(cracks_skeleton))
save_image(255 * cracks_skeleton_pruned / np.max(cracks_skeleton_pruned))
save_image(255 * cracks_skeleton_pruned_no_bp / np.max(cracks_skeleton_pruned_no_bp))
save_image(cracks_skeleton_pruned_no_bp_2_ep)
save_image(cracks_skeleton_restored)
return cracks_skeleton_restored
# import numpy for standard numerical calculations
import numpy as np
# read the image with mahotas as a grey image
img = m.imread('./testimg4.jpg', as_grey=True)
# read the image with mahotas again to obtain a color image where we can draw the ReebGraph in red (vertices) and green (edges)
imgColor = m.imread('./testimg4.jpg')
# Threshhold to remove artifacts from the jpg compression
img = (img > 100)
#get the dimensions of the image
x, y = np.shape(img)
#use the distance transform to obtain the distances per pixel of the medial axis
dmap = m.distance(img, metric='manhatten')
#use mathamatical morphology to obtain the medial axis (thinning function of mahotas)
skelImg = m.thin(img)
# draw the medial axis in the image
for idx, i in enumerate(skelImg):
for jdx, j in enumerate(i):
if skelImg[idx, jdx] == True:
imgColor[idx, jdx] = (255, 1, 1)
try:
imgColor[idx + 1, jdx] = (255, 1, 1)
except:
pass
imgColor[idx - 1, jdx] = (255, 1, 1)
try:
imgColor[idx, jdx + 1] = (255, 1, 1)
except:
pass
def skeletonisation(self):
#Skeletonisation by thinning
return mh.thin(self.imgArray)
self.rotatedIm=nd.rotate(self.particuleImage,majorAngle)
self.rotatedFlag=True
return majorAngle,compassTable,self.rotatedIm
user=os.path.expanduser("~")
#modify the path to your image
workdir=os.path.join(user,"Applications","ImagesTest","CytoProject","Jpp48","8","DAPI","particles")
file="part3.png"
complete_path=os.path.join(workdir,file)
if __name__ == "__main__":
im=readmagick.readimg(complete_path)
im0=np.copy(im)
hip=pymorph.subm(im,nd.gaussian_filter(im,5))
hip0=np.copy(hip)
im=mahotas.thin(im)
#im=mahotas.bwperim(im>0)
hip=mahotas.thin(hip)
#print im.dtype#print uint16
p1=particle(im)
print "particle 1",p1.cvxhull_area()
p2=particle(hip)
contour=mahotas.bwperim(im>0)
p3=particle(contour)
print "particle 1 hi pass",p2.cvxhull_area()
theta1,rosedesvents,VImage=p1.orientationByErosion(5)
theta2,rdv,VHip=p2.orientationByErosion(5)
x=rosedesvents[0,:]
y=rosedesvents[1,:]
xh=rdv[0,:]
yh=rdv[1,:]
# import numpy for standard numerical calculations
import numpy as np
# read the image with mahotas as a grey image
img=m.imread('./testimg4.jpg',as_grey=True)
# read the image with mahotas again to obtain a color image where we can draw the ReebGraph in red (vertices) and green (edges)
imgColor=m.imread('./testimg4.jpg')
# Threshhold to remove artifacts from the jpg compression
img=(img>100)
#get the dimensions of the image
x,y = np.shape(img)
#use the distance transform to obtain the distances per pixel of the medial axis
dmap = m.distance(img,metric='manhatten')
#use mathamatical morphology to obtain the medial axis (thinning function of mahotas)
skelImg=m.thin(img)
# draw the medial axis in the image
for idx,i in enumerate(skelImg):
for jdx,j in enumerate(i):
if skelImg[idx,jdx]==True:
imgColor[idx,jdx]=(255,1,1)
try:
imgColor[idx+1,jdx]=(255,1,1)
except:
pass
imgColor[idx-1,jdx]=(255,1,1)
try:
imgColor[idx,jdx+1]=(255,1,1)
except:pass
imgColor[idx,jdx-1]=(255,1,1)
def PST(I,
LPF=0.21,
Phase_strength=0.48,
Warp_strength=12.14,
Threshold_min=-1,
Threshold_max=0.0019,
Morph_flag=1):
# I: image
# Gaussian Low Pass Filter
# LPF = 0.21
# PST parameters:
# Phase_strength = 0.48
# Warp_strength = 12.14
# Thresholding parameters (for post processing after the edge is computed)
# Threshold_min = -1
# Threshold_max = 0.0019
# To compute analog edge, set Morph_flag = 0 and to compute digital edge, set Morph_flag = 1
# Morph_flag = 1
I_initial = I
if (len(I.shape) == 3):
I = I.mean(axis=2)
L = 0.5
x = np.linspace(-L, L, I.shape[0])
y = np.linspace(-L, L, I.shape[1])
[X1, Y1] = (np.meshgrid(x, y))
X = X1.T
Y = Y1.T
[THETA, RHO] = cart2pol(X, Y)
# Apply localization kernel to the original image to reduce noise
Image_orig_f = ((np.fft.fft2(I)))
expo = np.fft.fftshift(
np.exp(-np.power((np.divide(RHO, math.sqrt((LPF**2) /
np.log(2)))), 2)))
Image_orig_filtered = np.real(
np.fft.ifft2((np.multiply(Image_orig_f, expo))))
# Constructing the PST Kernel
PST_Kernel_1 = np.multiply(
np.dot(RHO, Warp_strength), np.arctan(np.dot(RHO, Warp_strength))
) - 0.5 * np.log(1 + np.power(np.dot(RHO, Warp_strength), 2))
PST_Kernel = PST_Kernel_1 / np.max(PST_Kernel_1) * Phase_strength
# Apply the PST Kernel
temp = np.multiply(np.fft.fftshift(np.exp(-1j * PST_Kernel)),
np.fft.fft2(Image_orig_filtered))
Image_orig_filtered_PST = np.fft.ifft2(temp)
# Calculate phase of the transformed image
PHI_features = np.angle(Image_orig_filtered_PST)
if Morph_flag == 0:
out = PHI_features
return out
else:
# find image sharp transitions by thresholding the phase
features = np.zeros((PHI_features.shape[0], PHI_features.shape[1]))
features[PHI_features > Threshold_max] = 1 # Bi-threshold decision
features[
PHI_features <
Threshold_min] = 1 # as the output phase has both positive and negative values
features[I < (
np.amax(I) / 20
)] = 0 # Removing edges in the very dark areas of the image (noise)
# apply binary morphological operations to clean the transformed image
out = features
out = mh.thin(out, 1)
out = mh.bwperim(out, 4)
out = mh.thin(out, 1)
out = mh.erode(out, np.ones((1, 1)))
Overlay = mh.overlay(I, out)
return (out, Overlay)
expo = np.fft.fftshift(np.exp(-np.power((np.divide(rho, math.sqrt((LPF ** 2) / np.log(2)))), 2)))
orig_filtered = np.real(np.fft.ifft2((np.multiply(orig, expo))))
PST_Kernel_1 = np.multiply(np.dot(rho, W), np.arctan(np.dot(rho, W))) - 0.5 * np.log(1 + np.power(np.dot(rho, W), 2))
PST_Kernel = PST_Kernel_1 / np.max(PST_Kernel_1) * S
temp = np.multiply(np.fft.fftshift(np.exp(-1j * PST_Kernel)), np.fft.fft2(orig_filtered))
temp = np.multiply(np.fft.fftshift(np.exp(-1j * PST_Kernel)), np.fft.fft2(Image_orig_filtered))
orig_filtered_PST = np.fft.ifft2(temp)
PHI_features = np.angle(Image_orig_filtered_PST)
features = np.zeros((PHI_features.shape[0], PHI_features.shape[1]))
features[PHI_features > Threshold_max] = 1
features[PHI_features < Threshold_min] = 1
features[I < (np.amax(I) / 20)] = 0
out = features
out = mh.thin(out, 1)
out = mh.bwperim(out, 4)
out = mh.thin(out, 1)
out = mh.erode(out, np.ones((1, 1)))
def phase_stretch_transform(img, LPF, S, W, threshold_min, threshold_max, flag):
L = 0.5
x = np.linspace(-L, L, img.shape[0])
y = np.linspace(-L, L, img.shape[1])
[X1, Y1] = (np.meshgrid(x, y))
X = X1.T
Y = Y1.T
theta, rho = cart2pol(X, Y)
orig = ((np.fft.fft2(img)))
expo = np.fft.fftshift(np.exp(-np.power((np.divide(rho, math.sqrt((LPF ** 2) / np.log(2)))), 2)))
orig_filtered = np.real(np.fft.ifft2((np.multiply(orig, expo))))
def python_nuvolatools(timg, tdrawable, mangaoptype=0, lowlevelnumber=0, highlevelnumber=255,
samplethreshold=90, minblobarea=1, maxblobarea=10, otsuon=0, fancyproc=0, cbrregistry=0, numberOfDilations=1):
####CBR Registry section
###note CBR registry is meant to become a very compact way of specifying multiple values (i.e. for multiple operations, even as binary values) and should be processed in this section to initialize the necessary variables. This has priority over everything else passed as arguments to the present function
####variables init
width = tdrawable.width
height = tdrawable.height
swidth = tdrawable.mask_bounds[2] - tdrawable.mask_bounds[0]
sheight = tdrawable.mask_bounds[3] - tdrawable.mask_bounds[1]
soffsetX = tdrawable.mask_bounds[0]
soffsetY = tdrawable.mask_bounds[1]
sselectionBox = tdrawable.mask_bounds
shapedetfillratio = 0.0
shapedetfillrange = 0.0
shapedethwratio = 0.0
shapedethwrange = 0.0
shapeselectex = 1
shapeinvertpic = 0
radiusoperation = 0
radiuspixel = 1
uniqueExtension = ""
if ((cbrregistry & 16)== 16):
dateNow = datetime.datetime.now()
uniqueExtension = str(time.mktime(dateNow.timetuple()))
if ((mangaoptype==2) or (mangaoptype==3) or (mangaoptype==4)):
if ((cbrregistry & 4)== 4):
extradata = readdialogdatafromshm()
root = Tk()
if ((cbrregistry & 4)!= 4):
d = MyDialog(root)
extradata = d.result
print "Extra parameters input result:", extradata
print "EXTRA PARAMETERS RAW", extradata
shapedetfillratio = float(extradata[0])
shapedethwratio = float(extradata[1])
shapedetfillrange = float(extradata[2])
shapedethwrange = float(extradata[3])
shapeselectex = int(extradata[4])
shapeinvertpic = int(extradata[5])
radiusoperation = int(extradata[6])
radiuspixel = int(extradata[7])
#basically, invertpic means setting the whitepores operation... so:
# (shapeinvertpic) complements the whitepores condition ~_~
scropBox = (swidth,sheight,soffsetX,soffsetY)
####creating work image (currently the imgg is NOT USED)
imgg = gimp.Image(width, height, GRAY)
imgg.disable_undo()
layer_imgg = gimp.Layer(imgg, "work layer", width, height, GRAY_IMAGE, 100, NORMAL_MODE)
layer_imgg.add_alpha()
imgg.add_layer(layer_imgg, 0)
pdb.gimp_image_crop(imgg,swidth,sheight,soffsetX,soffsetY)
##pdb.gimp_edit_copy(tdrawable)
##imggpastelayer = pdb.gimp_edit_paste(layer_imgg, True)
##imgg.add_layer(imggpastelayer, 1)
##imggreturnlayer = pdb.gimp_image_flatten(imgg)
wdrawable = tdrawable.copy()
if (fancyproc):
timg.add_layer(wdrawable, 0)
wdrawable.add_alpha()
pdb.gimp_layer_set_name(wdrawable,"nuvola work layer")
#### FANCY OPERATIONS (work layer)
if (fancyproc):
### this is a preset known to work well for me ~_~ ...doing two passes! (I should make this optonal tho)
## excluding the gaussian blur, useless for now.
#pdb.plug_in_gauss_rle(timg, wdrawable, maxblurrad***, 1, 1) #mablurrad will give error since this variable has been removed
pdb.gimp_levels(wdrawable, 0, 0, highlevelnumber, 1, 0, 255) #first let's minimize the background
pdb.plug_in_unsharp_mask(timg, wdrawable, 5, 0.5, 0) # only then, more crispness
#pdb.plug_in_unsharp_mask(timg, wdrawable, 5, 0.5, 0) ###maybe this is excessive, let's comment it
pdb.gimp_levels(wdrawable, 0, lowlevelnumber, 255, 1, 0, 255) #then, further darken the blacks
pdb.plug_in_antialias(timg, wdrawable) # a touch of antialias~
####conversion to numpy array
#pdb.file_png_save(timg, tdrawable, "/dev/shm/"+uniqueExtension+"nuvolatools-pngtimgfile.png", "pngtimgfile.png", 0, 9, 1, 1, 1, 1, 1)
#img = makenparrayfromfile("/dev/shm/"+uniqueExtension+"nuvolatools-pngtimgfile.png", sselectionBox, uniqueExtension)
#pdb.file_gif_save(timg, tdrawable, "/dev/shm/"+uniqueExtension+"nuvolatools-pngtimgfile.gif", "pngtimgfile.gif", 0, 0, 0, 0)
#img = makenparrayfromfile("/dev/shm/"+uniqueExtension+"nuvolatools-pngtimgfile.gif", sselectionBox, uniqueExtension)
pdb.file_tiff_save(timg, tdrawable, "/dev/shm/"+uniqueExtension+"nuvolatools-pngtimgfile.tif", "pngtimgfile.tif", 1)
print "calling nparrayfrom pngtimgfile"
img = makenparrayfromfile("/dev/shm/"+uniqueExtension+"nuvolatools-pngtimgfile.tif", sselectionBox, uniqueExtension)
#pdb.file_png_save(timg, wdrawable, "/dev/shm/"+uniqueExtension+"nuvolatools-wpngtimgfile.png", "wpngtimgfile.png", 0, 9, 1, 1, 1, 1, 1)
#wimg = makenparrayfromfile("/dev/shm/"+uniqueExtension+"nuvolatools-wpngtimgfile.png", sselectionBox, uniqueExtension) <-- only if fancyproc
if (fancyproc):
print "calling nparrayfrom wpngtimgfile"
pdb.file_tiff_save(timg, wdrawable, "/dev/shm/"+uniqueExtension+"nuvolatools-wpngtimgfile.tif", "wpngtimgfile.tif",1)
wimg = makenparrayfromfile("/dev/shm/"+uniqueExtension+"nuvolatools-wpngtimgfile.tif", sselectionBox, uniqueExtension)
#### thresholding and labeling
imglabel, labelnum, imgbin, imgbinneg, img, imgneg, imgotsu, imgtosaveotsu, imgotsuneg = imagepreprocesslabel(img, mangaoptype, otsuon, samplethreshold, shapeinvertpic)
if (fancyproc):
wimglabel, wlabelnum, wimgbin, wimgbinneg, wimg, wimgneg, wimgotsu, wimgtosaveotsu, wimgotsuneg = imagepreprocesslabel(wimg, mangaoptype, otsuon, samplethreshold, shapeinvertpic)
#### special for the shapedetector: we are actually using the work layer since it should give a much better shape separation
if ((mangaoptype==2) and (fancyproc)):
imglabel = wimglabel
labelnum = wlabelnum
imgbin = wimgbin
imgbinneg = wimgbinneg
img = wimg
imgneg = wimgneg
imgotsu = wimgotsu
imgtosaveotsu = wimgtosaveotsu
imgotsuneg = wimgotsuneg
####blob measurement via pymorph, parameter maxblobarea
pdb.gimp_progress_set_text('Measuring specks sizes.. (may take some time)')
imgblobdata = measurefromlabel(imglabel, tdrawable, fancyproc)
print imgblobdata, len(imgblobdata)
#len of data == number of labels, of course.
#### speckles operation start
if ((mangaoptype==0) or (mangaoptype==1)):
#print "making measurement from labels"
imgspeckles = img; #create a copy of the original nparray
imgspecklesres = makefromlabel(imgbinneg, imgspeckles, imglabel, imgblobdata,minblobarea,maxblobarea,fancyproc, shapedetfillratio, shapedetfillrange, shapedethwratio, shapedethwrange, shapeselectex, radiusoperation, radiuspixel, mangaoptype, uniqueExtension)
if (mangaoptype==2):
imgspeckles = img; #create a copy of the original nparray
### for now, fancyproc is needed true if we want to match specific shapes
fancyproc = True
print "pre-call shape det parameters", shapedetfillratio, shapedetfillrange, shapedethwratio, shapedethwrange, shapeselectex, radiusoperation, radiuspixel
imgspecklesres = makefromlabel(imgbinneg, imgspeckles, imglabel, imgblobdata,minblobarea,maxblobarea,fancyproc, shapedetfillratio, shapedetfillrange, shapedethwratio, shapedethwrange, shapeselectex, radiusoperation, radiuspixel, mangaoptype, uniqueExtension)
#### Fancy operation: dilation
#if (mangaoptype==1):
# propagate_mode=1
print "processing dilations"
for i in range(numberOfDilations):
imgspecklesresdil = mahotas.dilate(imgspecklesres)
imgspecklesres = imgspecklesresdil
#### Fancy operation: thinning skeleton from mahotas when processing lineart
print "thinning skeleton, this may take some minutes for very large pictures!"
if ((fancyproc) and (mangaoptype == 3)):
#if ((mangaoptype == 0)):
minsegment=256
minoverlap=64
#if (not fancyproc): # wimgbinneg is not declared if fancyproc is false, so for debugging purposes we assign it
# wimgbinneg = imgbinneg #preliminary results show a *less crisp* starting picture is MUCH desirable ~_~
wbinneglen=len(wimgbinneg)
wbinneglenintparts=wbinneglen/minsegment
wbinneglenintrem=wbinneglen-wbinneglenintparts*minsegment
wbinnegindex=0
#wimgbinneg=mahotas.erode(wimgbinneg) ###this has a very positive effect of stabilizing certain aspects of the picture, however it's also altering it greatly. Commented for the time being.
#wimgbinneg=mahotas.erode(wimgbinneg)
print "processing segment at", wbinnegindex, "/",wbinneglen
imgthin=mahotas.thin(wimgbinneg[wbinnegindex:wbinnegindex+minsegment])
wbinnegindex=wbinnegindex+minsegment
while (wbinnegindex<=wbinneglen):
pdb.gimp_progress_set_text("processing segment at "+str(wbinnegindex)+"/"+str(wbinneglen))
wbinnegindex=wbinnegindex-minoverlap ###makes it 25% more computationally expensive, but pays with no artifacts
if (wbinnegindex+minsegment <= wbinneglen):
imgthinpart=mahotas.thin(wimgbinneg[wbinnegindex:wbinnegindex+minsegment])
if (wbinnegindex+minsegment > wbinneglen):
imgthinpart=mahotas.thin(wimgbinneg[wbinnegindex:])
#print imgthin[0]
#print imgthinpart[0]
imgthin=np.append(imgthin[0:wbinnegindex+minoverlap/2],imgthinpart[minoverlap/2:], axis=0)
wbinnegindex=wbinnegindex+minsegment
print "skeleton thinned"
io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imgthin.png', imgthin*255)
### a word of wisdom: this damn fails with "memory error" for VERY large pictures ~_~
###skel, distance = medial_axis(wimgbinneg, return_distance=True)
###print "medialaxis done"
###skeldist=skel*distance
###io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-medialaxis2.png', skeldist)
#### if whitepores = True or (shapeinvertpic), invert the picture
if ((mangaoptype==1) or (shapeinvertpic)):
imgnegres = pymorph.neg(imgspecklesres)
imgspecklesres = imgnegres
####saving numpy intermediates
#io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imgtosaveotsu.png', imgtosaveotsu)
#io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imgbinneg.png', imgbinneg*255)
io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imgbin.png', imgbin*255)
if (otsuon == 1):
io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imgotsuneg.png',imgotsuneg*255)
io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imglabel.png',imglabel)
io.imsave('/dev/shm/'+uniqueExtension+'nuvolatools-imgspecklesres.png',imgspecklesres)
####creating spare GIMP layers
layer_specks = gimp.Layer(timg, "negative specks", swidth, sheight, GRAY_IMAGE, 100, NORMAL_MODE)
####loading specks as layer
layer_specks = pdb.gimp_file_load_layer(timg, '/dev/shm/'+uniqueExtension+'nuvolatools-imgspecklesres.png')
#pdb.gimp_layer_add_alpha(layer_specks)
timg.add_layer(layer_specks, 0)
layer_specks.add_alpha()
pdb.gimp_layer_set_name(layer_specks,"speckles remover")
#### if whitepores = True or (shapeinvertpic), use the correct layer name
if ((mangaoptype==1) or (shapeinvertpic)):
pdb.gimp_layer_set_name(layer_specks,"white pores filler")
print tdrawable.mask_bounds, tdrawable.mask_bounds[2]-tdrawable.mask_bounds[0], tdrawable.mask_bounds[3]-tdrawable.mask_bounds[1], tdrawable.mask_bounds[0], tdrawable.mask_bounds[1]
#### removing background and publishing
#layer_specks.resize(tdrawable.mask_bounds[2]-tdrawable.mask_bounds[0], tdrawable.mask_bounds[3]-tdrawable.mask_bounds[1], tdrawable.mask_bounds[0], tdrawable.mask_bounds[1])
pdb.gimp_by_color_select(layer_specks, gimpcolor.RGB(0,0,0), 0, CHANNEL_OP_REPLACE, False, False, 0, False)
#### if whitepores = True, invert the selection
if ((mangaoptype==1) or (shapeinvertpic)):
pdb.gimp_by_color_select(layer_specks, gimpcolor.RGB(255,255,255), 0, CHANNEL_OP_REPLACE, False, False, 0, False)
pdb.gimp_edit_clear(layer_specks)
pdb.gimp_selection_none(timg)
#print dir(layer_specks)
#print dir(pdb)
##layer_two = layer_one.copy()
##layer_two.mode = MULTIPLY_MODE
##layer_two.name = "Y Dots"
##timg.add_layer(layer_two, 0)
imgg.flatten()
##bump_layer = imgg.active_layer
layer_specks.translate(soffsetX, soffsetY)
####sending back layers
#layer_one.image = imgtosaveotsu
#timg.add_layer(layer_one, 0)
if ((cbrregistry & 1)== 1):
wdrawable.add_alpha()
pdb.gimp_selection_all(timg)
print "matched cbr registry 1 -> clearing wdrawable"
#pdb.gimp_drawable_delete(wdrawable)
pdb.gimp_edit_clear(wdrawable)
pdb.gimp_selection_none(timg)
if ((cbrregistry & 2)== 2):
print "saving current picture in xcf format"
timgfilename=pdb.gimp_image_get_filename(timg)
timgname=pdb.gimp_image_get_name(timg)
print timgname, timgfilename
timgfilenames=timgfilename+str(cbrregistry)+"op"+str(mangaoptype)+".xcf"
timgnames=timgname+str(cbrregistry)+"op"+str(mangaoptype)+".xcf"
print timgnames,timgfilenames
pdb.gimp_xcf_save(2,timg,wdrawable,timgfilenames,timgnames)
#pdb.file_xjt_save(timg,wdrawable,timgfilenames,timgnames,1,0,1,0)
#pdb.gimp_file_save(timg,wdrawable,timgfilenames,timgnames)
#### Final
if ((cbrregistry & 8)== 8):
os.system("rm -v /dev/shm/"+uniqueExtension+"nuvolatools-*")
os.system("rm -f /dev/shm/"+uniqueExtension+"nuvolatools-*")
gimp.delete(imgg)
#!/usr/bin/env python3 # -*- coding: utf-8 -*- """ Created on Tue Mar 13 23:26:47 2018 @author: raja """ import mahotas as mh img = np.array(img) im = img[:, 0:50, 0] im = im < 128 skel = mh.thin(im2) noholes = mh.morph.close_holes(skel) plt.subplot(311) plt.imshow(im) plt.subplot(312) plt.imshow(skel) plt.subplot(313) cskel = np.logical_not(skel) choles = np.logical_not(noholes) holes = np.logical_and(cskel, noholes) lab, n = mh.label(holes) print 'B has %s holes' % str(n) plt.imshow(lab)
def compute_skeleton(input_map):
'''Returns a skeleton image of the free space'''
return mahotas.thin(input_map)
return majorAngle, compassTable, self.rotatedIm
user = os.path.expanduser("~")
#modify the path to your image
workdir = os.path.join(user, "Applications", "ImagesTest", "CytoProject",
"Jpp48", "8", "DAPI", "particles")
file = "part3.png"
complete_path = os.path.join(workdir, file)
if __name__ == "__main__":
im = readmagick.readimg(complete_path)
im0 = np.copy(im)
hip = pymorph.subm(im, nd.gaussian_filter(im, 5))
hip0 = np.copy(hip)
im = mahotas.thin(im)
#im=mahotas.bwperim(im>0)
hip = mahotas.thin(hip)
#print im.dtype#print uint16
p1 = particle(im)
print "particle 1", p1.cvxhull_area()
p2 = particle(hip)
contour = mahotas.bwperim(im > 0)
p3 = particle(contour)
print "particle 1 hi pass", p2.cvxhull_area()
theta1, rosedesvents, VImage = p1.orientationByErosion(5)
theta2, rdv, VHip = p2.orientationByErosion(5)
x = rosedesvents[0, :]
y = rosedesvents[1, :]
xh = rdv[0, :]
yh = rdv[1, :]
def n_skeleton_branches(image):
"""Number of branches of skeleton."""
skeleton = mh.thin(image)
branches = end_points(skeleton)
return (sum(sum(branches != False)))
def compute_skeleton_map(boolean_map):
"""
Returns a skeletonised version of a binary image via thinning.
This makes use of the mahotas library.
"""
return mahotas.thin(boolean_map)
def compute_skeleton_map(boolean_map):
"""
Returns a skeletonised version of a binary image via thinning.
This makes use of the mahotas library.
"""
return mahotas.thin(boolean_map)
