def loadVertexDataFile(infile):
"""Loads the given Freesurfer vertex data, label, or annotation file.
This function return different things depending on what ``infile`` is:
- If ``infile`` is a vertex data file, a ``(nvertices,)`` array is
returned, containing one value for each vertex in the mesh.
- If ``infile`` is a ``mgh``/``mgz`` file, the image data is returned
as-is, with dimensions of length 1 squeezed out (under the assumption
that the image contains scalar vertex data).
- If ``infile`` is a vertex label file, a tuple containing the following
is returned:
- a ``(n,)`` array, containing the indices of all vertices that are
specified in the file.
- a ``(n,)`` array, containing scalar value for each vertex
- If ``infile`` is a vertex annotation file, a tuple containing the
following is returned:
- a ``(n,)`` array containing the indices of all ``n`` vertices that
are specified in the file.
- a ``(l, 5)`` array containing the RGBA colour, and the label value,
for every label that is specified in the file.
- A list of length ``l``, containing the names of every label that is
specified in the file.
"""
if isVertexDataFile(infile):
return nibfs.read_morph_data(infile)
elif isVertexLabelFile(infile):
return nibfs.read_label(infile, read_scalars=True)
elif isVertexAnnotFile(infile):
# nibabel 2.2.1 is broken w.r.t. .annot files.
# raise ValueError('.annot files are not yet supported')
labels, lut, names = nibfs.read_annot(infile, orig_ids=False)
return labels, lut, names
elif isVertexMGHFile(infile):
return fslmgh.MGHImage(infile)[:].squeeze()
else:
raise ValueError('Unrecognised freesurfer '
'file type: {}'.format(infile))
def backproject_label(label_file, subj, hemi):
"""Return label indices on individual subject surface."""
label_verts = fs.read_label(label_file)
# Define map of label on fsaverage surface
label = np.zeros(163842, np.int)
label[label_verts] = 1
# Reverse normalize and convert to vertex indices
subj_label = surface_transform(label, subj, hemi)
subj_label_verts = np.argwhere(subj_label).squeeze()
return subj_label_verts
def load_label(self, name):
"""Load in a Freesurfer .label file.
Label files are just text files indicating the vertices included
in the label. Each Surface instance has a dictionary of labels, keyed
by the name (which is taken from the file name if not given as an
argument.
"""
from nibabel import freesurfer
label = freesurfer.read_label(
path.join(self.data_path, 'label',
'%s.%s.label' % (self.hemi, name)))
label_array = np.zeros_like(self.x).astype(np.int)
label_array[label] = 1
self.labels[name] = label_array
def img2imgcoord_by_surf(self, target_subject, wf_base_dir=None, source_surface = 'pial', source_map_surface='pial', target_surface='pial'):
if wf_base_dir is None and self.working_dir is not None:
wf_base_dir = self.working_dir
elif wf_base_dir is None and self.working_dir is None:
print('Working dir has not been specified, results will be stored in: ', os.path.abspath('.'))
wf_base_dir = os.path.abspath('.')
rois,rois_paths = self.create_surf_roi(extents=2, wf_base_dir= wf_base_dir, wf_name='img2imgcoord_by_surf_roi', surface=source_surface, map_surface=source_map_surface, label2vol=False)
wf = pe.Workflow(name='label2label',base_dir=wf_base_dir)
for i in range(self.npoints):
l2l = Label2Label()
l2l.inputs.hemisphere = self.hemi[i]
l2l.inputs.subject_id = target_subject
l2l.inputs.sphere_reg = os.path.join(self.freesurfer_dir, target_subject, 'surf', self.hemi[i]+'.'+'sphere.reg')
l2l.inputs.white = os.path.join(self.freesurfer_dir, target_subject, 'surf', self.hemi[i]+'.'+'white')
l2l.inputs.source_subject = self.subject
l2l.inputs.source_label = rois_paths[i]
l2l.inputs.source_white = os.path.join(self.freesurfer_dir, self.subject, 'surf', self.hemi[i]+'.'+'white')
l2l.inputs.source_sphere_reg = os.path.join(self.freesurfer_dir, self.subject, 'surf', self.hemi[i]+'.'+'sphere.reg')
l2l.subjects_dir = self.freesurfer_dir
l2l_node = pe.Node(l2l,'label2label_{i}'.format(i=i))
wf.add_nodes([l2l_node])
try:
wf.run()
except RuntimeError:
pass
for i in range(self.npoints):
out_label_file = os.path.join(self.freesurfer_dir, target_subject, 'label', os.path.basename(rois_paths[i]).split('.label')[0]+'_converted'+'.label')
shutil.move(out_label_file, os.path.join(wf_base_dir, 'label2label','label2label_{i}'.format(i=i)))
new_coords = np.zeros((self.npoints,3))
for i in range(self.npoints):
label_file = os.path.join(wf_base_dir, 'label2label','label2label_{i}'.format(i=i),os.path.basename(rois_paths[i]).split('.label')[0]+'_converted'+'.label')
label_vertices = read_label(label_file)
label_vertices.sort()
label = Label(label_vertices,hemi=self.hemi[i],subject=target_subject)
vertex = label.center_of_mass()
targ_surf = FreesurferSurf(hemi=label.hemi, surf=target_surface, subject = target_subject, subjects_dir=self.freesurfer_dir)
new_coords[i,:] = targ_surf.get_coords(vertex)
return new_coords
def load_label(self, name):
"""Load in a Freesurfer .label file.
Label files are just text files indicating the vertices included
in the label. Each Surface instance has a dictionary of labels, keyed
by the name (which is taken from the file name if not given as an
argument.
"""
label = freesurfer.read_label(
path.join(self.data_path, 'label',
'%s.%s.label' % (self.hemi, name)))
label_array = np.zeros(len(self.x), np.int)
label_array[label] = 1
try:
self.labels[name] = label_array
except AttributeError:
self.labels = {name: label_array}
def load_data(self, filename):
""" Load the data from a surface scalar file
Parameters
----------
filename: str
Pathstr to a surface scalar file
Returns
-------
self: a Surface obejct
"""
if filename.endswith(('.curv', '.sulc', '.volume', '.thickness', '.area')):
data = np.expand_dims(freesurfer.read_morph_data(filename), axis=-1)
elif filename.endswith(('.shape.gii', '.func.gii')):
data = np.expand_dims(nib.load(filename).darrays[0].data, axis=-1)
elif filename.endswith(('.mgz', '.mgh')):
data = nib.load(filename).get_data()
data = data.reshape((data.shape[0], data.shape[-1]))
elif filename.endswith(('.dscalar.nii', '.dseries.nii')):
data = nib.load(filename).get_data()
data = data.T
elif filename.endswith('.label.gii'):
data = np.expand_dims(nib.load(filename).darrays[0].data, axis=-1)
elif filename.endswith('.dlabel.nii'):
data = nib.load(filename).get_data().T
elif filename.endswith('.label'):
data = np.expand_dims(freesurfer.read_label(filename), axis=-1)
elif filename.endswith('.annot'):
data, _, _ = freesurfer.read_annot(filename)
else:
suffix = os.path.split(filename)[1].split('.')[-1]
raise ImageFileError('This file format-{} is not supported at present.'.format(suffix))
self.data = data
def doSurfaceCorrelation():
'''
This method correlates two surfaces with one another within a certain
radius and writes out the result as a third surface file
The input format of all files should be mgh.
The mask should be an overlay of zeroes and ones.
Notes:
To run the whole thing on individual labels, we need to specify the
path to the label directory that contains the labels for the correct
hemisphere and template. Then, we can cut out the appropriate part and
store it in the file.
Testing:
- can I load the files that I have transformed with nibabel as morph files?
'''
# Stuff that should be defined dynamically elsewhere
gradientTemp = cf.gradientTemp
overlayTemp = cf.overlayTemp
maskTemp = cf.maskTemp
surfaceTemp = cf.templatePath
inputDir = cf.correlationInputDir
radii = cf.radii
hemispheres = cf.hemipsheres
score = cf.score
tempDir = cf.tempDir
outDir = cf.correlationOutDir
outName = cf.correlationOutName
useAbsVals = cf.useAbsVals
labelPath = cf.labelPath
doLabel = cf.doLabel
subjectList = loadSubjectList()
if doLabel:
# First, unpack the labels
findLabels()
# Load the label list
f = open(labelPath, 'rb')
labels = f.readlines()
f.close()
labelList = []
for line in labels:
label = line.strip()
labelList.append(label)
for hemi in hemispheres:
if hemi == 'lh':
altHemi = 'L'
elif hemi == 'rh':
altHemi = 'R'
else:
message = ('Your specified hemisphere (%s) is invalid. Ending' %
(hemi))
raise Exception(message)
# Load the template, we only need it once
surfacePath = (surfaceTemp % (hemi))
surface = sp.fileops.loadSurface(surfacePath)
for subID in subjectList:
subDir = os.path.join(inputDir, subID)
subOutDir = os.path.join(outDir, subID)
if not os.path.isdir(subOutDir):
os.makedirs(subOutDir)
# Generate the paths that we want to look at
gradientName = (gradientTemp % (subID, altHemi))
overlayName = (overlayTemp % (subID, hemi))
overlayName = ('%s.mgh' % overlayName)
overlayPath = os.path.join(subDir, overlayName)
maskPath = (maskTemp % (hemi))
# Check if we have the overlay
if not os.path.isfile(overlayPath):
message = ('Could not find overlay at %s.\nQuitting!' %
(overlayPath))
raise Exception(message)
# Check if we already have the gradient in mgh format
gradientMgh = ('%s.mgh' % gradientName)
gradientMghOutPath = os.path.join(subDir, gradientName)
gradientMghPath = os.path.join(subDir, gradientMgh)
if not os.path.isfile(gradientMghPath):
# Get the oneD file
gradientOneD = ('%s.1D' % (gradientName))
gradientOneDPath = os.path.join(subDir, gradientOneD)
if not os.path.isfile(gradientOneDPath):
message = (
'Could not find either 1D or mgh gradient in %s\n(%s / %s)'
% (subDir, gradientOneDPath, gradientMghPath))
raise Exception(message)
# Generate the mgh file
tempOneD = sp.fileops.loadVector(gradientOneDPath)
tempOutName = ('%s_temp' % gradientName)
tempOut = os.path.join(tempDir, tempOutName)
outStr = sp.fileops.writeVector(surface,
tempOneD,
mode='ascii')
savePath = sp.fileops.saveTxt(tempOut,
outStr,
'asc',
hemi=hemi)
sp.fileops.convertMorphAsciiMgh(savePath,
surfacePath,
outType='mgh',
outPath=gradientMghOutPath)
# Get the files loaded
gradient = sp.fileops.loadScalar(gradientMghPath)
overlay = sp.fileops.loadScalar(overlayPath)
if useAbsVals:
overlay = np.abs(overlay)
keepVerteces = sp.fileops.loadVector(maskPath, drop=2)
# Construct a graph to represent the surface
graph, numberVerteces = sp.procops.buildGraph(surface,
weighted=True)
# Truncate the graph based on the mask
truncGraph = sp.procops.keepNodes(graph, keepVerteces)
# For each radius, correlate the two values
for radius in radii:
# Generate the distance dictionary for the current radius
distanceDict = sp.procops.buildNeighbors(truncGraph, radius)
vertVec = sp.procops.slideRoiValues(numberVerteces,
keepVerteces,
distanceDict,
gradient,
morphVec2=overlay,
score=score)
# Generate the output paths
tempName = (outName % (subID, radius, hemi))
saveName = (outName % (subID, radius, hemi))
if doLabel:
# Get the label vector and take the average of the
# correlation map
#
# make a storage vector for the label processing
labelVec = np.zeros_like(vertVec)
for label in labelList:
# make label name
labelName = os.path.basename(label)
# read the label
labVec = nfs.read_label(label)
# use the indices to take a slice out of the overlay
sliceVec = vertVec[labVec]
# take the average of that slice
avgSlice = np.mean(sliceVec)
# and write it back into the appropriate verteces
labelVec[labVec] = avgSlice
# Adjust the names for the save files
tempName = ('%s_label' % tempName)
saveName = ('%s_label' % outName)
outVec = labelVec
else:
# If we are not using label computation, just write the
# vertex-vise vector
outVec = vertVec
# Generate the paths for the output
tempOut = os.path.join(tempDir, tempName)
saveOut = os.path.join(subOutDir, saveName)
# Generate the output files
outStr = sp.fileops.writeVector(surface, outVec, mode='ascii')
savePath = sp.fileops.saveTxt(tempOut,
outStr,
'asc',
hemi=hemi)
sp.fileops.convertMorphAsciiMgh(savePath,
surfacePath,
outType='mgh',
outPath=saveOut)
"""
The easiest way to label any vertex that could be in the region is with
add_label.
"""
brain.add_label("BA1", color="#A6BDDB")
"""
You can also threshold based on the probability of that region being at each
vertex.
"""
brain.add_label("BA1", color="#2B8CBE", scalar_thresh=.5)
"""
It's also possible to plot just the label boundary, in case you wanted to
overlay the label on an activation plot to asses whether it falls within that
region.
"""
brain.add_label("BA45", color="#F0F8FF", borders=3, scalar_thresh=.5)
brain.add_label("BA45", color="#F0F8FF", alpha=.3, scalar_thresh=.5)
"""
Finally, with a few tricks, you can display the whole probabilistic map.
"""
subjects_dir = environ["SUBJECTS_DIR"]
label_file = join(subjects_dir, "fsaverage", "label", "lh.BA6.label")
prob_field = np.zeros_like(brain._geo.x)
ids, probs = read_label(label_file, read_scalars=True)
prob_field[ids] = probs
brain.add_data(prob_field, thresh=1e-5, colormap="RdPu")
min=-.8,
max=.8,
colorbar=False)
"""
The easiest way to label any vertex that could be in the region is with
add_label.
"""
brain.add_label("BA1", color="#A6BDDB")
"""
You can also threshold based on the probability of that region being at each
vertex.
"""
brain.add_label("BA1", color="#2B8CBE", scalar_thresh=.5)
"""
It's also possible to plot just the label boundary, in case you wanted to
overlay the label on an activation plot to asses whether it falls within that
region.
"""
brain.add_label("BA45", color="#F0F8FF", borders=3, scalar_thresh=.5)
brain.add_label("BA45", color="#F0F8FF", alpha=.3, scalar_thresh=.5)
"""
Finally, with a few tricks, you can display the whole probabilistic map.
"""
subjects_dir = environ["SUBJECTS_DIR"]
label_file = join(subjects_dir, "fsaverage", "label", "lh.BA6.label")
prob_field = np.zeros_like(brain.geo['lh'].x)
ids, probs = read_label(label_file, read_scalars=True)
prob_field[ids] = probs
brain.add_data(prob_field, thresh=1e-5, colormap="RdPu")
def doSurfaceCorrelation():
'''
This method correlates two surfaces with one another within a certain
radius and writes out the result as a third surface file
The input format of all files should be mgh.
The mask should be an overlay of zeroes and ones.
Notes:
To run the whole thing on individual labels, we need to specify the
path to the label directory that contains the labels for the correct
hemisphere and template. Then, we can cut out the appropriate part and
store it in the file.
Testing:
- can I load the files that I have transformed with nibabel as morph files?
'''
# Stuff that should be defined dynamically elsewhere
gradientTemp = cf.gradientTemp
overlayTemp = cf.overlayTemp
maskTemp = cf.maskTemp
surfaceTemp = cf.templatePath
inputDir = cf.correlationInputDir
radii = cf.radii
hemispheres = cf.hemipsheres
score = cf.score
tempDir = cf.tempDir
outDir = cf.correlationOutDir
outName = cf.correlationOutName
useAbsVals = cf.useAbsVals
labelPath = cf.labelPath
doLabel = cf.doLabel
subjectList = loadSubjectList()
if doLabel:
# First, unpack the labels
findLabels()
# Load the label list
f = open(labelPath, 'rb')
labels = f.readlines()
f.close()
labelList = []
for line in labels:
label = line.strip()
labelList.append(label)
for hemi in hemispheres:
if hemi == 'lh':
altHemi = 'L'
elif hemi == 'rh':
altHemi = 'R'
else:
message = ('Your specified hemisphere (%s) is invalid. Ending' % (hemi))
raise Exception(message)
# Load the template, we only need it once
surfacePath = (surfaceTemp % (hemi))
surface = sp.fileops.loadSurface(surfacePath)
for subID in subjectList:
subDir = os.path.join(inputDir, subID)
subOutDir = os.path.join(outDir, subID)
if not os.path.isdir(subOutDir):
os.makedirs(subOutDir)
# Generate the paths that we want to look at
gradientName = (gradientTemp % (subID, altHemi))
overlayName = (overlayTemp % (subID, hemi))
overlayName = ('%s.mgh' % overlayName)
overlayPath = os.path.join(subDir, overlayName)
maskPath = (maskTemp % (hemi))
# Check if we have the overlay
if not os.path.isfile(overlayPath):
message = ('Could not find overlay at %s.\nQuitting!' % (overlayPath))
raise Exception(message)
# Check if we already have the gradient in mgh format
gradientMgh = ('%s.mgh' % gradientName)
gradientMghOutPath = os.path.join(subDir, gradientName)
gradientMghPath = os.path.join(subDir, gradientMgh)
if not os.path.isfile(gradientMghPath):
# Get the oneD file
gradientOneD = ('%s.1D' % (gradientName))
gradientOneDPath = os.path.join(subDir, gradientOneD)
if not os.path.isfile(gradientOneDPath):
message = ('Could not find either 1D or mgh gradient in %s\n(%s / %s)' % (subDir, gradientOneDPath, gradientMghPath))
raise Exception(message)
# Generate the mgh file
tempOneD = sp.fileops.loadVector(gradientOneDPath)
tempOutName = ('%s_temp' % gradientName)
tempOut = os.path.join(tempDir, tempOutName)
outStr = sp.fileops.writeVector(surface, tempOneD, mode='ascii')
savePath = sp.fileops.saveTxt(tempOut, outStr, 'asc',
hemi=hemi)
sp.fileops.convertMorphAsciiMgh(savePath, surfacePath,
outType='mgh',
outPath=gradientMghOutPath)
# Get the files loaded
gradient = sp.fileops.loadScalar(gradientMghPath)
overlay = sp.fileops.loadScalar(overlayPath)
if useAbsVals:
overlay = np.abs(overlay)
keepVerteces = sp.fileops.loadVector(maskPath, drop=2)
# Construct a graph to represent the surface
graph, numberVerteces = sp.procops.buildGraph(surface,
weighted=True)
# Truncate the graph based on the mask
truncGraph = sp.procops.keepNodes(graph, keepVerteces)
# For each radius, correlate the two values
for radius in radii:
# Generate the distance dictionary for the current radius
distanceDict = sp.procops.buildNeighbors(truncGraph, radius)
vertVec = sp.procops.slideRoiValues(numberVerteces, keepVerteces,
distanceDict, gradient,
morphVec2=overlay, score=score)
# Generate the output paths
tempName = (outName % (subID, radius, hemi))
saveName = (outName % (subID, radius, hemi))
if doLabel:
# Get the label vector and take the average of the
# correlation map
#
# make a storage vector for the label processing
labelVec = np.zeros_like(vertVec)
for label in labelList:
# make label name
labelName = os.path.basename(label)
# read the label
labVec = nfs.read_label(label)
# use the indices to take a slice out of the overlay
sliceVec = vertVec[labVec]
# take the average of that slice
avgSlice = np.mean(sliceVec)
# and write it back into the appropriate verteces
labelVec[labVec] = avgSlice
# Adjust the names for the save files
tempName = ('%s_label' % tempName)
saveName = ('%s_label' % outName)
outVec = labelVec
else:
# If we are not using label computation, just write the
# vertex-vise vector
outVec = vertVec
# Generate the paths for the output
tempOut = os.path.join(tempDir, tempName)
saveOut = os.path.join(subOutDir, saveName)
# Generate the output files
outStr = sp.fileops.writeVector(surface, outVec, mode='ascii')
savePath = sp.fileops.saveTxt(tempOut, outStr, 'asc',
hemi=hemi)
sp.fileops.convertMorphAsciiMgh(savePath, surfacePath,
outType='mgh',
outPath=saveOut)
