Esempio n. 1
0
	def __init__(self, out_dir, n, spacing_steps = 1, dry = False):
		#dry = True
		
		self.dry = dry
		self.out_dir = out_dir
		if n % 2 != 0:
			raise Exception('Center stacking requires even n')
		self.n = n
		self.n2 = n / 2
		# Specify in steps to be very exact instead of units
		self.spacing_steps = spacing_steps
		
		if self.dry:
			self.mc = DummyController()
			self.imager = DummyImager()
		else:
			self.mc = MC()
			self.mc.on()
			self.imager = PILImager()
Esempio n. 2
0
    def __init__(self, out_dir, n, spacing_steps=1, dry=False):
        #dry = True

        self.dry = dry
        self.out_dir = out_dir
        if n % 2 != 0:
            raise Exception('Center stacking requires even n')
        self.n = n
        self.n2 = n / 2
        # Specify in steps to be very exact instead of units
        self.spacing_steps = spacing_steps

        if self.dry:
            self.mc = DummyController()
            self.imager = DummyImager()
        else:
            self.mc = MC()
            self.mc.on()
            self.imager = PILImager()
Esempio n. 3
0
class Stacker:
    def __init__(self, out_dir, n, spacing_steps=1, dry=False):
        #dry = True

        self.dry = dry
        self.out_dir = out_dir
        if n % 2 != 0:
            raise Exception('Center stacking requires even n')
        self.n = n
        self.n2 = n / 2
        # Specify in steps to be very exact instead of units
        self.spacing_steps = spacing_steps

        if self.dry:
            self.mc = DummyController()
            self.imager = DummyImager()
        else:
            self.mc = MC()
            self.mc.on()
            self.imager = PILImager()

    def run(self):
        if os.path.exists(self.out_dir):
            raise Exception("dir %s already exists" % self.out_dir)
        os.mkdir(self.out_dir)

        self.mc.z.step(-self.n2 * self.spacing_steps)
        '''
		n = 0: 1 picture
		n = 2: 3 pictures
		'''
        for i in range(self.n + 1):
            self.imager.take_picture('%s/%03d__z%04fum.tif' %
                                     (self.out_dir, i, self.mc.z.get_um()))
            # Avoid moving at end
            if i != self.n:
                self.mc.z.step(self.spacing_steps)
                time.sleep(3)
        print 'Homing...'
        self.mc.z.home()
Esempio n. 4
0
class Stacker:
	def __init__(self, out_dir, n, spacing_steps = 1, dry = False):
		#dry = True
		
		self.dry = dry
		self.out_dir = out_dir
		if n % 2 != 0:
			raise Exception('Center stacking requires even n')
		self.n = n
		self.n2 = n / 2
		# Specify in steps to be very exact instead of units
		self.spacing_steps = spacing_steps
		
		if self.dry:
			self.mc = DummyController()
			self.imager = DummyImager()
		else:
			self.mc = MC()
			self.mc.on()
			self.imager = PILImager()

	def run(self):
		if os.path.exists(self.out_dir):
			raise Exception("dir %s already exists" % self.out_dir)
		os.mkdir(self.out_dir)
	
		self.mc.z.step(-self.n2 * self.spacing_steps)
		'''
		n = 0: 1 picture
		n = 2: 3 pictures
		'''
		for i in range(self.n + 1):
			self.imager.take_picture('%s/%03d__z%04fum.tif' % (self.out_dir, i, self.mc.z.get_um()))
			# Avoid moving at end
			if i != self.n:
				self.mc.z.step(self.spacing_steps)
				time.sleep(3)
		print 'Homing...'
		self.mc.z.home()