def setUp(self):
super().setUp()
self.gate = flow.PolygonOp(name = "Polygon",
xchannel = "V2-A",
ychannel = "Y2-A",
vertices = [(-95.86, 12436.45),
(116.29, 22530.75),
(767.63, 4873.08),
(101.64, 939.38),
(-266.93, 2914.59)])
def setUp(self):
self.cwd = os.path.dirname(
os.path.abspath(__file__)) + "/data/Plate01/"
tube1 = flow.Tube(file=self.cwd + 'RFP_Well_A3.fcs',
conditions={"Dox": 10.0})
tube2 = flow.Tube(file=self.cwd + 'CFP_Well_A4.fcs',
conditions={"Dox": 1.0})
import_op = flow.ImportOp(conditions={"Dox": "float"},
tubes=[tube1, tube2])
self.ex = import_op.apply()
self.gate = flow.PolygonOp(name="Polygon",
xchannel="V2-A",
ychannel="Y2-A",
vertices=[(-95.86, 12436.45),
(116.29, 22530.75),
(767.63, 4873.08),
(101.64, 939.38),
(-266.93, 2914.59)])
def setUp(self):
self.cwd = os.path.dirname(
os.path.abspath(__file__)) + "/data/Plate01/"
tube1 = flow.Tube(file=self.cwd + 'RFP_Well_A3.fcs',
conditions={"Dox": 10.0})
tube2 = flow.Tube(file=self.cwd + 'CFP_Well_A4.fcs',
conditions={"Dox": 1.0})
import_op = flow.ImportOp(conditions={"Dox": "float"},
tubes=[tube1, tube2])
ex = import_op.apply()
# this works so much better on transformed data
logicle = flow.LogicleTransformOp()
logicle.name = "Logicle transformation"
logicle.channels = ['V2-A', 'Y2-A', 'B1-A']
logicle.estimate(ex)
self.ex = logicle.apply(ex)
self.gate = flow.PolygonOp(name="Polygon",
xchannel="V2-A",
ychannel="Y2-A",
vertices=[(0, 0.6), (0.2, 0.8), (0.4, 0.6),
(0.2, 0.4)])