def setUp(self): super().setUp() self.gate = flow.PolygonOp(name = "Polygon", xchannel = "V2-A", ychannel = "Y2-A", vertices = [(-95.86, 12436.45), (116.29, 22530.75), (767.63, 4873.08), (101.64, 939.38), (-266.93, 2914.59)])
def setUp(self): self.cwd = os.path.dirname( os.path.abspath(__file__)) + "/data/Plate01/" tube1 = flow.Tube(file=self.cwd + 'RFP_Well_A3.fcs', conditions={"Dox": 10.0}) tube2 = flow.Tube(file=self.cwd + 'CFP_Well_A4.fcs', conditions={"Dox": 1.0}) import_op = flow.ImportOp(conditions={"Dox": "float"}, tubes=[tube1, tube2]) self.ex = import_op.apply() self.gate = flow.PolygonOp(name="Polygon", xchannel="V2-A", ychannel="Y2-A", vertices=[(-95.86, 12436.45), (116.29, 22530.75), (767.63, 4873.08), (101.64, 939.38), (-266.93, 2914.59)])
def setUp(self): self.cwd = os.path.dirname( os.path.abspath(__file__)) + "/data/Plate01/" tube1 = flow.Tube(file=self.cwd + 'RFP_Well_A3.fcs', conditions={"Dox": 10.0}) tube2 = flow.Tube(file=self.cwd + 'CFP_Well_A4.fcs', conditions={"Dox": 1.0}) import_op = flow.ImportOp(conditions={"Dox": "float"}, tubes=[tube1, tube2]) ex = import_op.apply() # this works so much better on transformed data logicle = flow.LogicleTransformOp() logicle.name = "Logicle transformation" logicle.channels = ['V2-A', 'Y2-A', 'B1-A'] logicle.estimate(ex) self.ex = logicle.apply(ex) self.gate = flow.PolygonOp(name="Polygon", xchannel="V2-A", ychannel="Y2-A", vertices=[(0, 0.6), (0.2, 0.8), (0.4, 0.6), (0.2, 0.4)])