def surrounding_OneMolecule_allframe(Input_MoleParentFolder, Output_moleParentFolder, Output_plotParentFolder, GroupNum, MoleculeNum, backbone_range = 2, close = False): GroupNum = int(GroupNum) MoleculeNum = int(MoleculeNum) fileContents = mfc.read_molecule_files_frominf(Input_MoleParentFolder, GroupNum, MoleculeNum) connect_count, frames, cells = mfc.get_frame_count_mole(fileContents) for FrameNum in frames: surrounding_OneMolecule(Input_MoleParentFolder, Output_moleParentFolder, Output_plotParentFolder, GroupNum, FrameNum, MoleculeNum, [-1,-1], backbone_range, close)
def simplify_lines_AllFrame(Input_MoleParentFolder_interval, GroupNum, MoleculeNum, threshold): fileContents = mfc.read_molecule_files_frominf(Input_MoleParentFolder_interval, GroupNum, MoleculeNum) connect_count, frames, cells = mfc.get_frame_count_mole(fileContents) straightness = [] for FrameNum in frames: ## readin molecule backbone information fileContents_setframe = mfc.get_content_setframe(fileContents, FrameNum) CellNumList_setframe = mfc.get_CellNumbers(fileContents_setframe) ## create a list to store turning points of simplify polyline list_knots = [0, len(CellNumList_setframe)-1] ## generate the simplify line simplify_test = simplify_line_test(CellNumList_setframe, threshold) while(simplify_test): ## for first recursion if len(list_knots) == 2: re_time = [0] simplify_line(CellNumList_setframe, 0, len(CellNumList_setframe)-1, list_knots, threshold, re_time) ## downstream recursion else: ## for each segment for i in range(0, len(list_knots) - 2): ## check whether this segment need more recursion if simplify_test_list[i]: re_time = [0] simplify_line(CellNumList_setframe, list_knots[i], list_knots[i+1], list_knots, threshold, re_time) list_knots = sorted(list(set(list_knots))) ## check every segment, decide simplify polyline creation is finished or not simplify_test_list = [] for i in range(0, len(list_knots) - 2): simplify_test_list.append(simplify_line_test(CellNumList_setframe[list_knots[i]:(list_knots[i+1] + 1)], threshold)) simplify_test = max(simplify_test_list) ## get turning positions turning_knots_cellnums = list(np.array(CellNumList_setframe)[list_knots]) ## get information related to turning angles theta_list = angle_list(turning_knots_cellnums) theta_max = max(map(abs, theta_list)) theta_sum = sum(map(abs, theta_list)) theta_net = sum(theta_list) ## estimation test_score_sum = float(1 / math.exp((abs(theta_net) + theta_max) / 2)) ## result report straightness.append([FrameNum, theta_net, theta_max, test_score_sum]) return straightness
def surrounding_OneMolecule_allframe(Input_MoleParentFolder,
Output_moleParentFolder,
Output_plotParentFolder,
GroupNum,
MoleculeNum,
backbone_range=2,
close=False):
GroupNum = int(GroupNum)
MoleculeNum = int(MoleculeNum)
fileContents = mfc.read_molecule_files_frominf(Input_MoleParentFolder,
GroupNum, MoleculeNum)
connect_count, frames, cells = mfc.get_frame_count_mole(fileContents)
for FrameNum in frames:
surrounding_OneMolecule(Input_MoleParentFolder,
Output_moleParentFolder,
Output_plotParentFolder, GroupNum, FrameNum,
MoleculeNum, [-1, -1], backbone_range, close)
def Effected_OneMolecule(Input_MoleParentFolder_interval, Input_SurroundingParentFolder_interval, GroupNum, MoleculeNum, layer, threshold):
#def main(argv):
# try:
# opts, args = getopt.getopt(argv, "hs:e:", ["help", "grammar="])
# except getopt.GetoptError:
# usage()
# sys.exit(2)
# GroupNum = int(args[0])
# MoleculeNum = int(args[1])
# layer = int(args[2])
GroupNum = int(GroupNum)
MoleculeNum = int(MoleculeNum)
layer = int(layer)
print GroupNum, MoleculeNum
GroupName = "group1-" + str(GroupNum) + "-inca34-outputs/"
GroupFolder = mfc.get_grouppath(GroupNum, Input_MoleParentFolder_interval)
if os.path.exists(GroupFolder):
molecule_fileContents = mfc.read_molecule_files_frominf(Input_MoleParentFolder_interval, GroupNum, MoleculeNum)
connect_count, frames, cells = mfc.get_frame_count_mole(molecule_fileContents)
for frame in frames:
BackbonefileContents = mfc.get_content_setframe(molecule_fileContents, frame)
FrameNum = frame
surrounding_fileName = "molecule" + str(MoleculeNum) + "_frame" + str(FrameNum) + ".txt"
SurroundingReadPath = os.path.join(Input_SurroundingParentFolder_interval, GroupName, surrounding_fileName)
SurrID = open(SurroundingReadPath, 'r')
SurroundingfileContents = SurrID.readlines()
ConditionList = determine_within_forbackbone(BackbonefileContents, SurroundingfileContents, layer, threshold)
## plotting
fig = plt.figure(frame)
BackboneIntensity = mfc.get_IntenAll(BackbonefileContents)
attention_x = list(map(multiply, range(0, len(BackboneIntensity)), ConditionList))
attention_x = filter(lambda x:x is not 0, attention_x)
if ConditionList[0] != 0:
attention_x.insert(0, 0)
attention_y = map(multiply, BackboneIntensity, ConditionList)
if attention_y.count(0) != 0:
attention_y = filter(lambda y:y is not 0, attention_y)
line, = plt.plot(BackboneIntensity, label = "intensity porfile")
dot, = plt.plot(attention_x, attention_y, "ro", color = "red")
Title = "Group" + str(GroupNum) + "_Molecule" + str(MoleculeNum) + " Backbone1 intensity" + "(default threshold" + str(threshold) + ")"
plt.title(Title)
plt.xlabel("position(orientation depend on alignment)/pixels")
plt.ylabel("intensity")
# plt.legend([line], "intensity porfile", loc = 4)
plt.legend([line, dot], ["intensity porfile", "effected pixels(from layer"+str(layer) + ")"])
# print ConditionList
plt.show()
else:
print "group not exist!"
def simplify_lines_AllFrame(Input_MoleParentFolder_interval, GroupNum,
MoleculeNum, threshold):
fileContents = mfc.read_molecule_files_frominf(
Input_MoleParentFolder_interval, GroupNum, MoleculeNum)
connect_count, frames, cells = mfc.get_frame_count_mole(fileContents)
straightness = []
for FrameNum in frames:
## readin molecule backbone information
fileContents_setframe = mfc.get_content_setframe(
fileContents, FrameNum)
CellNumList_setframe = mfc.get_CellNumbers(fileContents_setframe)
## create a list to store turning points of simplify polyline
list_knots = [0, len(CellNumList_setframe) - 1]
## generate the simplify line
simplify_test = simplify_line_test(CellNumList_setframe, threshold)
while (simplify_test):
## for first recursion
if len(list_knots) == 2:
re_time = [0]
simplify_line(CellNumList_setframe, 0,
len(CellNumList_setframe) - 1, list_knots,
threshold, re_time)
## downstream recursion
else:
## for each segment
for i in range(0, len(list_knots) - 2):
## check whether this segment need more recursion
if simplify_test_list[i]:
re_time = [0]
simplify_line(CellNumList_setframe, list_knots[i],
list_knots[i + 1], list_knots, threshold,
re_time)
list_knots = sorted(list(set(list_knots)))
## check every segment, decide simplify polyline creation is finished or not
simplify_test_list = []
for i in range(0, len(list_knots) - 2):
simplify_test_list.append(
simplify_line_test(
CellNumList_setframe[list_knots[i]:(list_knots[i + 1] +
1)], threshold))
simplify_test = max(simplify_test_list)
## get turning positions
turning_knots_cellnums = list(
np.array(CellNumList_setframe)[list_knots])
## get information related to turning angles
theta_list = angle_list(turning_knots_cellnums)
theta_max = max(map(abs, theta_list))
theta_sum = sum(map(abs, theta_list))
theta_net = sum(theta_list)
## estimation
test_score_sum = float(1 / math.exp((abs(theta_net) + theta_max) / 2))
## result report
straightness.append([FrameNum, theta_net, theta_max, test_score_sum])
return straightness
def Effected_OneMolecule(Input_MoleParentFolder_interval,
Input_SurroundingParentFolder_interval, GroupNum,
MoleculeNum, layer, threshold):
#def main(argv):
# try:
# opts, args = getopt.getopt(argv, "hs:e:", ["help", "grammar="])
# except getopt.GetoptError:
# usage()
# sys.exit(2)
# GroupNum = int(args[0])
# MoleculeNum = int(args[1])
# layer = int(args[2])
GroupNum = int(GroupNum)
MoleculeNum = int(MoleculeNum)
layer = int(layer)
print GroupNum, MoleculeNum
GroupName = "group1-" + str(GroupNum) + "-inca34-outputs/"
GroupFolder = mfc.get_grouppath(GroupNum, Input_MoleParentFolder_interval)
if os.path.exists(GroupFolder):
molecule_fileContents = mfc.read_molecule_files_frominf(
Input_MoleParentFolder_interval, GroupNum, MoleculeNum)
connect_count, frames, cells = mfc.get_frame_count_mole(
molecule_fileContents)
for frame in frames:
BackbonefileContents = mfc.get_content_setframe(
molecule_fileContents, frame)
FrameNum = frame
surrounding_fileName = "molecule" + str(
MoleculeNum) + "_frame" + str(FrameNum) + ".txt"
SurroundingReadPath = os.path.join(
Input_SurroundingParentFolder_interval, GroupName,
surrounding_fileName)
SurrID = open(SurroundingReadPath, 'r')
SurroundingfileContents = SurrID.readlines()
ConditionList = determine_within_forbackbone(
BackbonefileContents, SurroundingfileContents, layer,
threshold)
## plotting
fig = plt.figure(frame)
BackboneIntensity = mfc.get_IntenAll(BackbonefileContents)
attention_x = list(
map(multiply, range(0, len(BackboneIntensity)), ConditionList))
attention_x = filter(lambda x: x is not 0, attention_x)
if ConditionList[0] != 0:
attention_x.insert(0, 0)
attention_y = map(multiply, BackboneIntensity, ConditionList)
if attention_y.count(0) != 0:
attention_y = filter(lambda y: y is not 0, attention_y)
line, = plt.plot(BackboneIntensity, label="intensity porfile")
dot, = plt.plot(attention_x, attention_y, "ro", color="red")
Title = "Group" + str(GroupNum) + "_Molecule" + str(
MoleculeNum
) + " Backbone1 intensity" + "(default threshold" + str(
threshold) + ")"
plt.title(Title)
plt.xlabel("position(orientation depend on alignment)/pixels")
plt.ylabel("intensity")
# plt.legend([line], "intensity porfile", loc = 4)
plt.legend([line, dot], [
"intensity porfile",
"effected pixels(from layer" + str(layer) + ")"
])
# print ConditionList
plt.show()
else:
print "group not exist!"
