def __create_worklist_series(self):
# Generates the buffer transfer worklists.
self.add_debug('Create worklist series ...')
if self.create_pool_racks:
pool_buf_vol = get_pool_buffer_volume()
prep_trf_vol = get_preparation_plate_transfer_volume(
preparation_plate_volume=
self.preparation_plate_volume)
prep_buf_vol = self.preparation_plate_volume - prep_trf_vol
else:
pool_buf_vol = None
prep_buf_vol = \
self.preparation_plate_volume \
- self.__stock_transfer_volume * self.number_designs
generator = LibraryCreationWorklistGenerator(
self.__base_layout,
self.__stock_concentration,
self.library_name,
prep_buf_vol,
pool_buffer_volume=pool_buf_vol,
parent=self)
self.__worklist_series = generator.get_result()
if self.__worklist_series is None:
msg = 'Error when trying to generate worklist series.'
self.add_error(msg)
def __create_worklist_series(self):
# Generates the buffer transfer worklists.
self.add_debug("Create worklist series ...")
if self.create_pool_racks:
pool_buf_vol = get_pool_buffer_volume()
prep_trf_vol = get_preparation_plate_transfer_volume(preparation_plate_volume=self.preparation_plate_volume)
prep_buf_vol = self.preparation_plate_volume - prep_trf_vol
else:
pool_buf_vol = None
prep_buf_vol = self.preparation_plate_volume - self.__stock_transfer_volume * self.number_designs
generator = LibraryCreationWorklistGenerator(
self.__base_layout,
self.__stock_concentration,
self.library_name,
prep_buf_vol,
pool_buffer_volume=pool_buf_vol,
parent=self,
)
self.__worklist_series = generator.get_result()
if self.__worklist_series is None:
msg = "Error when trying to generate worklist series."
self.add_error(msg)
def __write_pool_creation_section(self):
# This is the stock transfer part (creating pools from single molecule
# designs).
self.add_debug('Writing pool section.')
self._write_headline(header_text=self.HEADER_POOL_CREATION,
preceding_blank_lines=0)
lines = []
volume_line = self.VOLUME_LINE \
% get_pool_transfer_volume(number_designs=
self.iso.iso_request.number_designs)
volume_line += ' each'
lines.append(volume_line)
buffer_line = self.BUFFER_LINE % get_pool_buffer_volume()
lines.append(buffer_line)
for sector_index in sorted(self.single_stock_rack_map.keys()):
lines.append('')
lines.append(self.QUADRANT_LINE % (sector_index + 1))
barcodes = self.single_stock_rack_map[sector_index]
target_rack = self.pool_stock_rack_map[sector_index]
src_line = self.SOURCE_LINE_PLURAL % (', '.join(sorted(barcodes)))
lines.append(src_line)
lines.append(self.TARGET_LINE % (target_rack))
self._write_body_lines(lines)
def __write_pool_creation_section(self):
# This is the stock transfer part (creating pools from single molecule
# designs).
self.add_debug('Writing pool section.')
self._write_headline(header_text=self.HEADER_POOL_CREATION,
preceding_blank_lines=0)
lines = []
volume_line = self.VOLUME_LINE \
% get_pool_transfer_volume(number_designs=
self.iso.iso_request.number_designs)
volume_line += ' each'
lines.append(volume_line)
buffer_line = self.BUFFER_LINE % get_pool_buffer_volume()
lines.append(buffer_line)
for sector_index in sorted(self.single_stock_rack_map.keys()):
lines.append('')
lines.append(self.QUADRANT_LINE % (sector_index + 1))
barcodes = self.single_stock_rack_map[sector_index]
target_rack = self.pool_stock_rack_map[sector_index]
src_line = self.SOURCE_LINE_PLURAL % (', '.join(sorted(barcodes)))
lines.append(src_line)
lines.append(self.TARGET_LINE % (target_rack))
self._write_body_lines(lines)
