'cleaning protocol',
sample_prep_method='Plates Clean.setup', # thorough cleaning
itc_method='water5inj.inj',
analysis_method='Control',
experimental_conditions=dict(target_temperature=25,
equilibration_time=60,
stir_rate=1000,
reference_power=5),
injections=5 *
[dict(volume_inj=7.5, duration_inj=15, spacing=150, filter_period=5)],
)
# Define ITC Experiment.
# use specified protocol by default
itc_experiment_set = ITCExperimentSet(
name='Bovine carbonic anhydrase (CAII) experiments')
# Add available plates for experiments.
destination_plate = Labware(RackLabel='DestinationPlate', RackType='ITC Plate')
itc_experiment_set.addDestinationPlate(destination_plate)
nreplicates = 1 # number of replicates of each experiment
# Add cleaning experiment.
name = 'initial cleaning water titration'
itc_experiment_set.addExperiment(
ITCExperiment(name=name,
syringe_source=water_trough,
cell_source=water_trough,
protocol=cleaning_protocol,
cell_volume=cell_volume))
binding_protocol = ITCProtocol(
'1:1 binding protocol',
sample_prep_method='Plates Standard.setup',
itc_method='Chodera_HSA.inj', # TODO Define new protocol with more injections?
analysis_method='Onesite') # TODO better default analysis?
# Protocol for cleaning protocol
binding_cleaning_protocol = ITCProtocol(
'cleaning protocol',
sample_prep_method='Plates Clean.setup',
itc_method='Chodera_HSA.inj',
analysis_method='Onesite')
# Define ITC Experiment.
# use specified protocol by default
itc_experiment_set = ITCExperimentSet(name='Human Serum Albumin experiments')
# Add available plates for experiments.
itc_experiment_set.addDestinationPlate(
Labware(
RackLabel='DestinationPlate',
RackType='ITC Plate'))
itc_experiment_set.addDestinationPlate(
Labware(
RackLabel='DestinationPlate2',
RackType='ITC Plate'))
nreplicates = 1 # number of replicates of each experiment
# Add water control titrations.
for replicate in range(1):
itc_method='ChoderaWaterWater.inj',
analysis_method='Control',
experimental_conditions=dict(target_temperature=25,
equilibration_time=60,
stir_rate=1000,
reference_power=5),
injections=[
dict(volume_inj=0.2, duration_inj=0.4, spacing=60, filter_period=0.5)
] + 10 *
[dict(volume_inj=3.0, duration_inj=6, spacing=120, filter_period=0.5)],
)
# Define ITC Experiment.
# use specified protocol by default
itc_experiment_set = ITCExperimentSet(name='Receptor-ligand experiments')
# Add available plates for experiments.
number_of_itc_plates = 2 # number of ITC plates to use (up to 4)
for plate_index in range(number_of_itc_plates):
destination_plate = Labware(RackLabel=f'DestinationPlate{plate_index+1}',
RackType='ITC Plate')
itc_experiment_set.addDestinationPlate(destination_plate)
# Add cleaning experiment.
name = 'initial cleaning water titration'
itc_experiment_set.addExperiment(
ITCExperiment(name=name,
syringe_source=water_trough,
cell_source=water_trough,
protocol=cleaning_protocol,
cell_volume=cell_volume))
'cleaning protocol',
sample_prep_method='Plates Clean.setup',
itc_method='water5inj.inj',
analysis_method='Control',
experimental_conditions=dict(target_temperature=25,
equilibration_time=60,
stir_rate=1000,
reference_power=5),
injections=5 *
[dict(volume_inj=7.5, duration_inj=15, spacing=150, filter_period=5)],
)
# Define ITC Experiment.
# use specified protocol by default
itc_experiment_set = ITCExperimentSet(name='HDR')
# Add available plates for experiments.
itc_experiment_set.addDestinationPlate(
Labware(RackLabel='DestinationPlate', RackType='ITC Plate'))
itc_experiment_set.addDestinationPlate(
Labware(RackLabel='DestinationPlate2', RackType='ITC Plate'))
nreplicates = 1 # number of replicates of each experiment
# Add cleaning experiment.
name = 'initial cleaning water titration'
itc_experiment_set.addExperiment(
ITCExperiment(
name=name,
syringe_source=water_trough,
cell_source=water_trough,
'cleaning protocol',
sample_prep_method='Plates Clean.setup',
itc_method='water5inj.inj',
analysis_method='Control',
experimental_conditions=dict(target_temperature=25,
equilibration_time=60,
stir_rate=1000,
reference_power=5),
injections=5 *
[dict(volume_inj=7.5, duration_inj=15, spacing=150, filter_period=5)],
)
# Define ITC Experiment.
# use specified protocol by default
itc_experiment_set = ITCExperimentSet(name='SAMPL4-CB7 host-guest experiments')
# Add available plates for experiments.
itc_experiment_set.addDestinationPlate(
Labware(RackLabel='DestinationPlate', RackType='ITC Plate'))
itc_experiment_set.addDestinationPlate(
Labware(RackLabel='DestinationPlate2', RackType='ITC Plate'))
nreplicates = 1 # number of replicates of each experiment
# Add cleaning experiment.
name = 'initial cleaning water titration'
itc_experiment_set.addExperiment(
ITCExperiment(
name=name,
syringe_source=water_trough,
cell_source=water_trough,
'cleaning protocol',
sample_prep_method='Plates Clean.setup', # thorough cleaning
itc_method='water5inj.inj',
analysis_method='Control',
experimental_conditions=dict(target_temperature=25,
equilibration_time=60,
stir_rate=1000,
reference_power=5),
injections=5 *
[dict(volume_inj=7.5, duration_inj=15, spacing=150, filter_period=5)],
)
# Define ITC Experiment.
# use specified protocol by default
itc_experiment_set = ITCExperimentSet(
name='Bovine carbonic anhydrase (CAII) and PHIPA pilot experiments')
# Add available plates for experiments.
destination_plate = Labware(RackLabel='DestinationPlate', RackType='ITC Plate')
itc_experiment_set.addDestinationPlate(destination_plate)
nreplicates = 1 # number of replicates of each experiment
# Add cleaning experiment.
name = 'initial cleaning water titration'
itc_experiment_set.addExperiment(
ITCExperiment(name=name,
syringe_source=water_trough,
cell_source=water_trough,
protocol=cleaning_protocol,
cell_volume=cell_volume))
