buffer_masses = {'Compound 10': 10.065 * ureg.gram}
compound_masses = {'Compound 10': 15.05 * ureg.milligram}
ligand_solutions = list()
for (index, ligand) in enumerate(ligands):
ligand_solution = SimpleSolution(
compound=ligand,
compound_mass=compound_masses[ligand.name],
solvent=buffer,
solvent_mass=buffer_masses[ligand.name],
location=PipettingLocation(source_plate.RackLabel,
source_plate.RackType, index + 1))
ligand_solutions.append(ligand_solution)
# For convenience, report concentrations
print('STOCK SOLUTION CONCENTRATIONS:')
location = ITCExperimentSet.human_readable(receptor_solution.location)
print(
f"{receptor_solution.name:20} in {location:32} : {receptor_solution.compound_mass.to('milligrams')} receptor in {receptor_solution.solvent_mass.to('grams')} solvent : {receptor_solution.concentration.to('millimolar'):30}"
)
for ligand_solution in ligand_solutions:
location = ITCExperimentSet.human_readable(ligand_solution.location)
print(
f"{ligand_solution.name:20} in {location:32} : {ligand_solution.compound_mass.to('milligrams')} compound in {ligand_solution.solvent_mass.to('grams')} solvent : {ligand_solution.concentration.to('millimolar'):30}"
)
print('')
# Define ITC protocol.
# Receptor cell concentrations to evaluate
#cell_concentrations = [0.010 * ureg.millimolar, 0.020 * ureg.millimolar, 0.030 * ureg.millimolar]
#cell_concentrations = [0.020 * ureg.millimolar]
