def test_primer_Design_saving_to_text_file():
files_ = [dedent(x) for x in
('''\
>fw64 t-sequence
atgactgctaacccttc
>rv64 t-sequence
catcgtaagtttcgaac''',
'''\
>fw64 t-sequence
atgactgctaacccttcN
>rv64 t-sequence
catcgtaagtttcgaacN''',
'''\
>fw64 t-sequence
atgactgctaacccttcN''',
'''\
>fw64 t-sequence
atgactgctaacccttcN
>rv64 t-sequence
catcgtaagtttcgaac''',
'''\
>rv64 t-sequence
catcgtaagtttcgaacN''',
'''\
>rv64 t-sequence
catcgtaagtttcgaacN
>fw64 t-sequence
atgactgctaacccttc''',
'''\
>fw64 t-sequenceZ
atgactgctaacccttc
>rv64 t-sequenceZ
catcgtaagtttcgaac''',
'''\
>fw64 t-sequenceZ
atgactgctaacccttc
>rv64 t-sequenceZ
catcgtaagtttcgaac
>fw64 t-sequence
atgactgctaacccttc
>rv64 t-sequence
catcgtaagtttcgaac''',
'''\
>fw64x t-sequence
atgactgctaacccttc
>rv64x t-sequence
catcgtaagtttcgaac''',
'''\
>fw64x t-sequence
atgactgctaacccttc
>rv64x t-sequence
catcgtaagtttcgaac
>fw64 t-sequence
atgactgctaacccttc
>rv64 t-sequence
catcgtaagtttcgaac''',
)]
templ=Dseqrecord("atgactgctaacccttccttggtgttgaacaagatcgacgacatttcgttcgaaacttacgatg")
templ.accession = "t-sequence"
try:
os.remove("PRIMERS.TXT")
except OSError:
pass
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
assert os.path.isfile("PRIMERS.TXT")
with open("PRIMERS.TXT", "r") as f: text=f.read().strip()
assert text == files_[0]
with open("PRIMERS.TXT", "w") as f: text=f.write(files_[1])
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
with open("PRIMERS.TXT", "r") as f: text=f.read().strip()
assert text == files_[1]
with open("PRIMERS.TXT", "w") as f: text=f.write(files_[2])
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
with open("PRIMERS.TXT", "r") as f: text=f.read().strip()
assert text == files_[3]
with open("PRIMERS.TXT", "w") as f: text=f.write(files_[4])
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
with open("PRIMERS.TXT", "r") as f: text=f.read().strip()
assert text == files_[5]
with open("PRIMERS.TXT", "w") as f: text=f.write(files_[6])
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
with open("PRIMERS.TXT", "r") as f: text=f.read().strip()
assert text == files_[7]
with open("PRIMERS.TXT", "w") as f: text=f.write(files_[8])
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
with open("PRIMERS.TXT", "r") as f: text=f.read().strip()
assert text == files_[9]
with open("PRIMERS.TXT", "w") as f: text=f.write('')
pf, pr = cloning_primers(templ, path="PRIMERS.TXT")
f, r = parse("PRIMERS.TXT", ds=False)
assert f.description == "fw64 t-sequence"
assert r.description == "rv64 t-sequence"
