Пример #1
0
    def getElements(self, node_id, x, y, x_label=None, y_label=None):

        e = NodeDecorator.getElements(self, node_id, x, y)

        if x_label is None:
            x_label = x
        if y_label is None:
            y_label = y

        t = self.mTree.node(node_id).data.taxon
        species = self.mExtractSpecies(t)

        if species not in self.mMapSpecies2Colour:
            self.mMapSpecies2Colour[species] = COLOURS[len(
                self.mMapSpecies2Colour) % len(COLOURS)]

        if species in self.mMapSpecies2Name:
            tx = re.sub(species, "%s" % self.mMapSpecies2Name[species], t)
        else:
            tx = t

        colour = self.getColour(node_id, x, y)

        if self.mPlotLabel:
            ee = SVGdraw.text(x_label,
                              y_label,
                              tx,
                              self.mFontSize,
                              self.mFont,
                              stroke="rgb(%i,%i,%i)" % colour,
                              text_anchor="left")

            if self.mMapTaxon2URL is not None:
                url = self.mMapTaxon2URL(t)
                if url:
                    l = SVGdraw.link(url)
                    l.addElement(ee)
                    e.append(l)
                else:
                    e.append(ee)
            else:
                e.append(ee)
        return e
Пример #2
0
    def getElements(self, node_id, x, y, x_label=None, y_label=None):

        e = NodeDecorator.getElements(self, node_id, x, y)

        if x_label is None:
            x_label = x
        if y_label is None:
            y_label = y

        t = self.mTree.node(node_id).data.taxon
        species = self.mExtractSpecies(t)

        if species not in self.mMapSpecies2Colour:
            self.mMapSpecies2Colour[species] = COLOURS[
                len(self.mMapSpecies2Colour) % len(COLOURS)]

        if species in self.mMapSpecies2Name:
            tx = re.sub(species, "%s" % self.mMapSpecies2Name[species], t)
        else:
            tx = t

        colour = self.getColour(node_id, x, y)

        if self.mPlotLabel:
            ee = SVGdraw.text(x_label, y_label,
                              tx,
                              self.mFontSize,
                              self.mFont,
                              stroke="rgb(%i,%i,%i)" % colour,
                              text_anchor="left")

            if self.mMapTaxon2URL is not None:
                url = self.mMapTaxon2URL(t)
                if url:
                    l = SVGdraw.link(url)
                    l.addElement(ee)
                    e.append(l)
                else:
                    e.append(ee)
            else:
                e.append(ee)
        return e
Пример #3
0
    def addDuplication(self, entries, map_gene2pos, height,
                       url=None,
                       with_separator=True,
                       link_to_previous=False,
                       quality2symbol={},
                       quality2mask={}):
        """add a dot in row/col."""

        mi, ma = None, 0

        pos = bisect.bisect(self.mColourThresholds, height)
        master_colour = self.mColours[pos]

        chrs = {}
        points = []

        if not link_to_previous:
            self.mPreviousPoints = {}

        ########################################################
        ########################################################
        ########################################################
        # convert gene list to a set of points
        ########################################################
        for species, transcript, gene, quality in entries:

            chr, strand, first_res, last_res = map_gene2pos[gene]
            chrs[chr] = 1
            pos1 = self.getPosition(chr, strand, first_res)
            pos2 = self.getPosition(chr, strand, last_res)

            a = min(pos1, pos2)
            b = max(pos1, pos2)

            if mi is None:
                mi = a
            else:
                mi = min(a, mi)
            ma = max(b, ma)

            points.append((pos1, pos2, gene, quality, chr))

        ########################################################
        ########################################################
        ########################################################
        # decide whether we need to increment the radius
        ########################################################
        cis = len(chrs) == 1

        old_radius = self.mRadius
        is_overlap = False
        if cis:
            if not self.mLastChr:
                self.mLastChr = chr

            if chr != self.mLastChr:
                self.mRadius = self.mRadiusFallBack
                self.mLastMax = ma
                self.mPreviousMax = ma
                self.mLastChr = chr
            else:
                if self.mPreviousMax + self.mMinDistance > mi:
                    # overlap due to close proximitiy
                    self.mRadius += self.mRadiusIncrement
                    if with_separator:
                        self.addSeparator()

                    # true overlap
                    if self.mPreviousMax > mi:
                        is_overlap = True

                elif self.mLastMax + self.mMinDistance > mi:
                    pass
                else:
                    self.mRadius = self.mRadiusFallBack

                self.mLastMax = max(self.mLastMax, ma)
        else:
            if self.mLastMax > mi:
                self.mRadius += self.mRadiusIncrement
                if with_separator:
                    self.addSeparator()

        self.mPreviousMin = mi
        self.mPreviousMax = ma
        self.mPreviousCis = cis

        self.mRadiusMax = max(self.mRadius, self.mRadiusMax)

        ########################################################
        ########################################################
        ########################################################
        # draw points
        ########################################################
        link_colour = master_colour
        link_rad_width = self.mLinkRadStrokeWidth
        link_arc_width = self.mLinkArcStrokeWidth
        new_points = {}
        for pos1, pos2, gene, quality, chr in points:

            angle = self.getAngle((pos1 + pos2) / 2)

            x, y = self.getPosOnArc(angle, self.mRadius)

            try:
                symbol = quality2symbol[quality]
            except KeyError:
                symbol = "rect"

            if quality in quality2mask:
                colour = self.mLinkColourSymbolMasked
                link_colour = self.mLinkColourMasked
                link_rad_width = self.mLinkStrokeWidthMasked
                link_arc_width = self.mLinkStrokeWidthMasked
            else:
                colour = master_colour

            if gene in self.mPreviousPoints:
                continue

            new_points[gene] = (x, y, angle, quality, chr)

            if symbol == "circle":
                ee = SVGdraw.circle(x, y, self.mLinkSymbolSize,
                                    fill="rgb(%i,%i,%i)" % colour,
                                    stroke="black",
                                    stroke_width=self.mLinkStrokeWidthSymbol)
            elif symbol == "rect":
                ee = SVGdraw.rect(x - self.mLinkSymbolSize / 2, y - self.mLinkSymbolSize / 2,
                                  self.mLinkSymbolSize, self.mLinkSymbolSize,
                                  fill="rgb(%i,%i,%i)" % colour,
                                  stroke="black",
                                  stroke_width=self.mLinkStrokeWidthSymbol)

            if url:
                e = SVGdraw.link(url % gene)
                e.addElement(ee)
            else:
                e = ee

            self.addWheelElement(e)

        ########################################################
        ########################################################
        ########################################################
        # write all arcs in between old points and new points
        # cis:   circular arc
        # trans: radial arc
        ########################################################

        angles = []

        for x1, y1, angle1, quality1, chr1 in new_points.values():

            # reduce clutter by not writing arc to the same angle
            for x2, y2, angle2, quality2, chr2 in self.mPreviousPoints.values():

                for a in angles:
                    if a - self.mAngleResolution < angle2 < a + self.mAngleResolution:
                        break
                else:
                    angles.append(angle2)

                    d = SVGdraw.pathdata(x1, y1)

                    if chr1 == chr2:
                        d.relellarc(
                            self.mRadius, self.mRadius, 0, 0, 1, x2 - x1, y2 - y1)
                        link_width = link_rad_width
                    else:
                        d.relellarc(
                            self.mRadius * 2, self.mRadius * 2, 0, 0, 0, x2 - x1, y2 - y1)
                        link_width = link_arc_width

                    e = SVGdraw.path(d,
                                     fill="none",
                                     stroke="rgb(%i,%i,%i)" % link_colour,
                                     stroke_width=link_width)

                    self.addWheelElement(e, self.mPlaneLinks)

        # plot lines between new points
        new_genes = new_points.keys()

        for g1 in range(len(new_genes) - 1):

            x1, y1, angle1, quality1, chr1 = new_points[new_genes[g1]]

            for g2 in range(g1 + 1, len(new_genes)):

                x2, y2, angle2, quality2, chr2 = new_points[new_genes[g2]]

                for a in angles:
                    if a - self.mAngleResolution < angle2 < a + self.mAngleResolution:
                        break
                else:
                    angles.append(angle2)

                    d = SVGdraw.pathdata(x1, y1)

                    if chr1 == chr2:
                        d.relellarc(
                            self.mRadius, self.mRadius, 0, 0, 1, x2 - x1, y2 - y1)
                        link_width = link_rad_width
                    else:
                        d.relellarc(
                            self.mRadius * 2, self.mRadius * 2, 0, 0, 0, x2 - x1, y2 - y1)
                        link_width = link_arc_width

                    e = SVGdraw.path(d,
                                     fill="none",
                                     stroke="rgb(%i,%i,%i)" % link_colour,
                                     stroke_width=link_width)

                    self.addWheelElement(e, self.mPlaneLinks)

        # add new points to old points
        for k, v in new_points.items():
            self.mPreviousPoints[k] = v
Пример #4
0
    def addDuplication(self,
                       entries,
                       map_gene2pos,
                       height,
                       url=None,
                       with_separator=True,
                       link_to_previous=False,
                       quality2symbol={},
                       quality2mask={}):
        """add a dot in row/col."""

        mi, ma = None, 0

        pos = bisect.bisect(self.mColourThresholds, height)
        master_colour = self.mColours[pos]

        chrs = {}
        points = []
        for species, transcript, gene, quality in entries:

            chr, strand, first_res, last_res = map_gene2pos[gene]
            chrs[chr] = 1
            pos1 = self.getPosition(chr, strand, first_res)
            pos2 = self.getPosition(chr, strand, last_res)

            a = min(pos1, pos2)
            b = max(pos1, pos2)

            if mi is None:
                mi = a
            else:
                mi = min(a, mi)
            ma = max(b, ma)

            points.append((pos1, pos2, gene, quality))

        # decide whether we need to increment the radius
        cis = len(chrs) == 1

        old_radius = self.mRadius
        is_overlap = False
        if cis:
            if not self.mLastChr:
                self.mLastChr = chr

            if chr != self.mLastChr:
                self.mRadius = self.mRadiusFallBack
                self.mLastMax = ma
                self.mPreviousMax = ma
                self.mLastChr = chr
            else:
                if self.mPreviousMax + self.mMinDistance > mi:
                    # overlap due to close proximitiy
                    self.mRadius += self.mRadiusIncrement
                    if with_separator:
                        self.addSeparator()

                    # true overlap
                    if self.mPreviousMax > mi:
                        is_overlap = True

                elif self.mLastMax + self.mMinDistance > mi:
                    pass
                else:
                    self.mRadius = self.mRadiusFallBack

                self.mLastMax = max(self.mLastMax, ma)
        else:
            if self.mLastMax > mi:
                self.mRadius += self.mRadiusIncrement
                if with_separator:
                    self.addSeparator()

        if cis and self.mPreviousCis and (link_to_previous or is_overlap):

            # print old_x1, old_y1, old_x2, old_y2, new_x1, new_y2, new_x2,
            # new_y2
            r1 = old_radius
            r2 = self.mRadius
            old_x1, old_y1 = self.getPosOnArcForRadius(self.mPreviousMin, r1)
            old_x2, old_y2 = self.getPosOnArcForRadius(self.mPreviousMax, r1)
            new_x1, new_y1 = self.getPosOnArcForRadius(mi, r2)
            new_x2, new_y2 = self.getPosOnArcForRadius(ma, r2)

            if link_to_previous:

                # print cis, entries, chrs

                d = SVGdraw.pathdata(old_x1, old_y1)
                d.ellarc(r1, r1, 0, 0, 1, old_x2, old_y2)
                d.line(new_x2, new_y2)
                d.ellarc(r2, r2, 0, 0, 1, new_x1, new_y1)
                d.line(old_x1, old_y1)

                e = SVGdraw.path(d,
                                 fill="rgb(%i,%i,%i)" % GREY,
                                 stroke="rgb(%i,%i,%i)" % GREY,
                                 stroke_width=1)

            else:

                # get points of center
                old_x1, old_y1 = self.getPosOnArcForRadius(
                    (self.mPreviousMax + self.mPreviousMin) / 2, r1)
                new_x1, new_y1 = self.getPosOnArcForRadius((ma + mi) / 2, r2)

                # lines for interleaved spans: skip
                if not ((self.mPreviousMin < mi and self.mPreviousMax > ma) or
                        (self.mPreviousMin > mi and self.mPreviousMax < ma)):
                    ##                     d = SVGdraw.pathdata( old_x2, old_y2 )
                    ##                     d.line( new_x2, new_y2 )
                    ##                     d.move( new_x1, new_y1 )
                    ##                     d.line( old_x1, old_y1 )
                    ##                     d.move( old_x2, old_y2 )
                    # e = SVGdraw.path( d,
                    ##                                       fill = "none",
                    ##                                       stroke = "rgb(%i,%i,%i)" % BLACK,
                    # stroke_width = self.mMarkerWidth / 2 )

                    e = SVGdraw.line(old_x1,
                                     old_y1,
                                     new_x1,
                                     new_y1,
                                     fill="none",
                                     stroke="rgb(%i,%i,%i)" % GREY,
                                     stroke_width=self.mMarkerWidth / 2)
                else:
                    # lines for covering spans, as these overlaps
                    e = None


# e = SVGdraw.line( old_x1, old_y1,
##                                       new_x1, new_y1,
##                                       fill = "none",
##                                       stroke = "rgb(%i,%i,%i)" % GREY,
# stroke_width = self.mMarkerWidth / 2 )

            if e:
                self.addElement(e, self.mPlaneJoins)

        self.mPreviousMin = mi
        self.mPreviousMax = ma
        self.mPreviousCis = cis

        self.mRadiusMax = max(self.mRadius, self.mRadiusMax)

        # draw points
        link_colour = master_colour
        link_width = 10
        for pos1, pos2, gene, quality in points:

            angle = self.getAngle((pos1 + pos2) / 2)

            x, y = self.getPosOnArc(angle, self.mRadius)

            try:
                symbol = quality2symbol[quality]
            except KeyError:
                symbol = "rect"

            if quality in quality2mask:
                colour = GREY
                link_colour = GREY
                link_width = 1
            else:
                colour = master_colour

            if symbol == "circle":
                ee = SVGdraw.circle(x,
                                    y,
                                    self.mMarkerWidth,
                                    fill="rgb(%i,%i,%i)" % colour,
                                    stroke="black",
                                    stroke_width=1)
            elif symbol == "rect":
                ee = SVGdraw.rect(x - self.mMarkerWidth / 2,
                                  y - self.mMarkerWidth / 2,
                                  self.mMarkerWidth,
                                  self.mMarkerWidth,
                                  fill="rgb(%i,%i,%i)" % colour,
                                  stroke="black",
                                  stroke_width=1)

            if url:
                e = SVGdraw.link(url % gene)
                e.addElement(ee)
            else:
                e = ee
            self.addElement(e)

        angle1 = self.getAngle(mi)
        angle2 = self.getAngle(ma)

        x1, y1 = self.getPosOnArc(angle1, self.mRadius)
        x2, y2 = self.getPosOnArc(angle2, self.mRadius)

        d = SVGdraw.pathdata(x1, y1)
        if cis:
            d.ellarc(self.mRadius, self.mRadius, 0, 0, 1, x2, y2)
        else:
            d.ellarc(self.mRadius * 2, self.mRadius * 2, 0, 0, 0, x2, y2)

        e = SVGdraw.path(d,
                         fill="none",
                         stroke="rgb(%i,%i,%i)" % link_colour,
                         stroke_width=link_width)

        self.addElement(e)
Пример #5
0
    def plotGenes(self):
        """plot orthologs orthologwise."""

        # sort all entries by coordinates
        # and build coordinates
        map_gene2coord = {}

        y = self.mHeaderHeight

        self.mMapGene2Coord = {}

        reference_genes = None

        for species in self.mSpeciesList:
            genes = self.getGenes(species)
            genes.sort(lambda x, y: cmp((x.contig, x.mFrom), (y.contig, y.mFrom)))

            if reference_genes:
                genes = self.getSortedGenes(genes, reference_genes)
            else:
                genes = self.getSortedGenesByBlockSize(genes)

            x = self.getRowWidth(genes)

            x = self.mHeaderWidth + (self.mDataWidth - x) / 2

            last_contig, last_end = None, 0

            # build list of genes to skip (note: this can be improved
            # by working in blocks from the start.)
            blocks = self.getBlocks(genes)

            for block in blocks:

                nlinks = 0
                for gene in block:
                    nlinks += len(self.mMapGroup2Genes[gene.mOrthologId])
                if nlinks == len(block):
                    continue

                x = self.addGroupStart(x, y, gene.contig, gene.mFrom)

                # count number of links to be made
                for gene in block:

                    self.mMapGene2Coord[gene] = (x, y)

                    ee = self.plotGene(x, y, gene)

                    if self.mGeneUrl:
                        e = SVGdraw.link(self.mGeneUrl % gene)
                        e.addElement(ee)
                    else:
                        e = ee

                    self.addElement(e)
                    x += self.mBlockSize + self.mVerticalSeparator

                    last_contig, last_end = gene.contig, gene.mTo

            y += self.mBlockSize + self.mHorizontalSeparator

            # set orientation
            if self.mOrientation == "local":
                reference_genes = genes
            elif self.mOrientation == "first":
                if not reference_genes:
                    reference_genes = genes
Пример #6
0
    def plotGenes(self):
        """plot orthologs orthologwise."""

        ## sort all entries by coordinates
        ## and build coordinates
        map_gene2coord = {}

        y = self.mHeaderHeight

        self.mMapGene2Coord = {}

        reference_genes = None

        for species in self.mSpeciesList:
            genes = self.getGenes(species)
            genes.sort(lambda x, y: cmp((x.contig, x.mFrom),
                                        (y.contig, y.mFrom)))

            if reference_genes:
                genes = self.getSortedGenes(genes, reference_genes)
            else:
                genes = self.getSortedGenesByBlockSize(genes)

            x = self.getRowWidth(genes)

            x = self.mHeaderWidth + (self.mDataWidth - x) / 2

            last_contig, last_end = None, 0

            # build list of genes to skip (note: this can be improved
            # by working in blocks from the start.)
            blocks = self.getBlocks(genes)

            for block in blocks:

                nlinks = 0
                for gene in block:
                    nlinks += len(self.mMapGroup2Genes[gene.mOrthologId])
                if nlinks == len(block): continue

                x = self.addGroupStart(x, y, gene.contig, gene.mFrom)

                # count number of links to be made
                for gene in block:

                    self.mMapGene2Coord[gene] = (x, y)

                    ee = self.plotGene(x, y, gene)

                    if self.mGeneUrl:
                        e = SVGdraw.link(self.mGeneUrl % gene)
                        e.addElement(ee)
                    else:
                        e = ee

                    self.addElement(e)
                    x += self.mBlockSize + self.mVerticalSeparator

                    last_contig, last_end = gene.contig, gene.mTo

            y += self.mBlockSize + self.mHorizontalSeparator

            ## set orientation
            if self.mOrientation == "local":
                reference_genes = genes
            elif self.mOrientation == "first":
                if not reference_genes:
                    reference_genes = genes
Пример #7
0
    def addDuplication( self, entries, map_gene2pos, height,
                        url = None,
                        with_separator=True,
                        link_to_previous = False,
                        quality2symbol = {},
                        quality2mask = {}):
        """add a dot in row/col."""
        
        mi, ma = None, 0

        pos = bisect.bisect( self.mColourThresholds, height )
        master_colour = self.mColours[pos]
        
        chrs = {}
        points = []
        for species, transcript, gene, quality in entries:

            chr, strand, first_res, last_res = map_gene2pos[gene]
            chrs[chr] = 1
            pos1 = self.getPosition( chr, strand, first_res )
            pos2 = self.getPosition( chr, strand, last_res )

            a = min( pos1, pos2 )
            b = max( pos1, pos2 )

            if mi == None:
                mi = a
            else:
                mi = min(a, mi)
            ma = max(b, ma)
            
            points.append( (pos1, pos2, gene, quality) )

        ## decide whether we need to increment the radius
        cis = len(chrs) == 1

        old_radius = self.mRadius
        is_overlap = False
        if cis:
            if not self.mLastChr:
                self.mLastChr = chr

            if chr != self.mLastChr:
                self.mRadius = self.mRadiusFallBack
                self.mLastMax = ma
                self.mPreviousMax = ma
                self.mLastChr = chr
            else:
                if self.mPreviousMax + self.mMinDistance > mi:
                    ## overlap due to close proximitiy
                    self.mRadius += self.mRadiusIncrement
                    if with_separator: self.addSeparator()

                    ## true overlap
                    if self.mPreviousMax > mi:
                        is_overlap = True
                    
                elif self.mLastMax + self.mMinDistance > mi:
                    pass
                else:
                    self.mRadius = self.mRadiusFallBack
                    
                self.mLastMax = max(self.mLastMax, ma)
        else:
            if self.mLastMax > mi:
                self.mRadius += self.mRadiusIncrement
                if with_separator: self.addSeparator()
                
        if cis and self.mPreviousCis and (link_to_previous or is_overlap):

            # print old_x1, old_y1, old_x2, old_y2, new_x1, new_y2, new_x2, new_y2
            r1 = old_radius
            r2 = self.mRadius
            old_x1, old_y1 = self.getPosOnArcForRadius( self.mPreviousMin, r1 )
            old_x2, old_y2 = self.getPosOnArcForRadius( self.mPreviousMax, r1 )
            new_x1, new_y1 = self.getPosOnArcForRadius( mi, r2 )
            new_x2, new_y2 = self.getPosOnArcForRadius( ma, r2 )

            if link_to_previous:
                
                # print cis, entries, chrs
                
                d = SVGdraw.pathdata( old_x1, old_y1 )
                d.ellarc( r1, r1, 0, 0, 1, old_x2, old_y2 )            
                d.line( new_x2, new_y2 )
                d.ellarc( r2, r2, 0, 0, 1, new_x1, new_y1 )
                d.line( old_x1, old_y1 )
                
                e = SVGdraw.path( d,
                                  fill = "rgb(%i,%i,%i)" % GREY,
                                  stroke = "rgb(%i,%i,%i)" % GREY,
                                  stroke_width = 1 )
                
            else:

                # get points of center
                old_x1, old_y1 = self.getPosOnArcForRadius( (self.mPreviousMax + self.mPreviousMin) / 2, r1 )
                new_x1, new_y1 = self.getPosOnArcForRadius( (ma + mi) / 2, r2 )

                # lines for interleaved spans: skip
                if not ((self.mPreviousMin < mi and self.mPreviousMax > ma) or \
                        (self.mPreviousMin > mi and self.mPreviousMax < ma)) :
##                     d = SVGdraw.pathdata( old_x2, old_y2 )
##                     d.line( new_x2, new_y2 )
##                     d.move( new_x1, new_y1 )
##                     d.line( old_x1, old_y1 )
##                     d.move( old_x2, old_y2 )
##                     e = SVGdraw.path( d,
##                                       fill = "none",
##                                       stroke = "rgb(%i,%i,%i)" % BLACK,
##                                       stroke_width = self.mMarkerWidth / 2 )

                    e = SVGdraw.line( old_x1, old_y1,
                                      new_x1, new_y1,
                                      fill = "none",
                                      stroke = "rgb(%i,%i,%i)" % GREY,
                                      stroke_width = self.mMarkerWidth / 2 )
                else:
                    # lines for covering spans, as these overlaps
                    e = None
##                     e = SVGdraw.line( old_x1, old_y1,
##                                       new_x1, new_y1,
##                                       fill = "none",
##                                       stroke = "rgb(%i,%i,%i)" % GREY,
##                                       stroke_width = self.mMarkerWidth / 2 )

            if e:
                self.addElement( e, self.mPlaneJoins )
                    

        self.mPreviousMin = mi
        self.mPreviousMax = ma
        self.mPreviousCis = cis
        
        self.mRadiusMax = max(self.mRadius, self.mRadiusMax)
        
        ## draw points
        link_colour = master_colour
        link_width = 10
        for pos1, pos2, gene, quality in points:
            
            angle = self.getAngle( (pos1 + pos2) / 2 )
            
            x,y = self.getPosOnArc( angle, self.mRadius )

            try:
                symbol = quality2symbol[quality]
            except KeyError:
                symbol = "rect"

            if quality in quality2mask:
                colour = GREY
                link_colour = GREY
                link_width = 1
            else:
                colour = master_colour

            if symbol == "circle":
                ee = SVGdraw.circle( x, y, self.mMarkerWidth,
                                     fill = "rgb(%i,%i,%i)" % colour,
                                     stroke="black",
                                     stroke_width= 1)
            elif symbol == "rect":
                ee =  SVGdraw.rect( x-self.mMarkerWidth/2, y-self.mMarkerWidth/2,
                                    self.mMarkerWidth, self.mMarkerWidth,
                                    fill = "rgb(%i,%i,%i)" % colour,
                                    stroke="black",
                                    stroke_width= 1)

            if url:
                e = SVGdraw.link( url % gene )
                e.addElement( ee )
            else:
                e = ee
            self.addElement( e )                


        angle1 = self.getAngle( mi ) 
        angle2 = self.getAngle( ma )

        x1,y1 = self.getPosOnArc( angle1, self.mRadius )
        x2,y2 = self.getPosOnArc( angle2, self.mRadius )

        d = SVGdraw.pathdata( x1, y1 )
        if cis:
            d.ellarc( self.mRadius, self.mRadius, 0, 0, 1, x2, y2 )            
        else:
            d.ellarc( self.mRadius * 2, self.mRadius * 2, 0, 0, 0, x2, y2 )

        e = SVGdraw.path( d,
                          fill = "none",
                          stroke = "rgb(%i,%i,%i)" % link_colour,
                          stroke_width = link_width )

        self.addElement(e)
Пример #8
0
    def addDuplication(self, entries, map_gene2pos, height,
                       url=None,
                       with_separator=True,
                       link_to_previous=False,
                       quality2symbol={},
                       quality2mask={}):
        """add a dot in row/col."""

        mi, ma = None, 0

        pos = bisect.bisect(self.mColourThresholds, height)
        master_colour = self.mColours[pos]

        chrs = {}
        points = []

        if not link_to_previous:
            self.mPreviousPoints = {}

        ########################################################
        ########################################################
        ########################################################
        # convert gene list to a set of points
        ########################################################
        for species, transcript, gene, quality in entries:

            chr, strand, first_res, last_res = map_gene2pos[gene]
            chrs[chr] = 1
            pos1 = self.getPosition(chr, strand, first_res)
            pos2 = self.getPosition(chr, strand, last_res)

            a = min(pos1, pos2)
            b = max(pos1, pos2)

            if mi is None:
                mi = a
            else:
                mi = min(a, mi)
            ma = max(b, ma)

            points.append((pos1, pos2, gene, quality, chr))

        ########################################################
        ########################################################
        ########################################################
        # decide whether we need to increment the radius
        ########################################################
        cis = len(chrs) == 1

        old_radius = self.mRadius
        is_overlap = False
        if cis:
            if not self.mLastChr:
                self.mLastChr = chr

            if chr != self.mLastChr:
                self.mRadius = self.mRadiusFallBack
                self.mLastMax = ma
                self.mPreviousMax = ma
                self.mLastChr = chr
            else:
                if self.mPreviousMax + self.mMinDistance > mi:
                    # overlap due to close proximitiy
                    self.mRadius += self.mRadiusIncrement
                    if with_separator:
                        self.addSeparator()

                    # true overlap
                    if self.mPreviousMax > mi:
                        is_overlap = True

                elif self.mLastMax + self.mMinDistance > mi:
                    pass
                else:
                    self.mRadius = self.mRadiusFallBack

                self.mLastMax = max(self.mLastMax, ma)
        else:
            if self.mLastMax > mi:
                self.mRadius += self.mRadiusIncrement
                if with_separator:
                    self.addSeparator()

        self.mPreviousMin = mi
        self.mPreviousMax = ma
        self.mPreviousCis = cis

        self.mRadiusMax = max(self.mRadius, self.mRadiusMax)

        ########################################################
        ########################################################
        ########################################################
        # draw points
        ########################################################
        link_colour = master_colour
        link_rad_width = self.mLinkRadStrokeWidth
        link_arc_width = self.mLinkArcStrokeWidth
        new_points = {}
        for pos1, pos2, gene, quality, chr in points:

            angle = self.getAngle((pos1 + pos2) / 2)

            x, y = self.getPosOnArc(angle, self.mRadius)

            try:
                symbol = quality2symbol[quality]
            except KeyError:
                symbol = "rect"

            if quality in quality2mask:
                colour = self.mLinkColourSymbolMasked
                link_colour = self.mLinkColourMasked
                link_rad_width = self.mLinkStrokeWidthMasked
                link_arc_width = self.mLinkStrokeWidthMasked
            else:
                colour = master_colour

            if gene in self.mPreviousPoints:
                continue

            new_points[gene] = (x, y, angle, quality, chr)

            if symbol == "circle":
                ee = SVGdraw.circle(x, y, self.mLinkSymbolSize,
                                    fill="rgb(%i,%i,%i)" % colour,
                                    stroke="black",
                                    stroke_width=self.mLinkStrokeWidthSymbol)
            elif symbol == "rect":
                ee = SVGdraw.rect(x - self.mLinkSymbolSize / 2, y - self.mLinkSymbolSize / 2,
                                  self.mLinkSymbolSize, self.mLinkSymbolSize,
                                  fill="rgb(%i,%i,%i)" % colour,
                                  stroke="black",
                                  stroke_width=self.mLinkStrokeWidthSymbol)

            if url:
                e = SVGdraw.link(url % gene)
                e.addElement(ee)
            else:
                e = ee

            self.addWheelElement(e)

        ########################################################
        ########################################################
        ########################################################
        # write all arcs in between old points and new points
        # cis:   circular arc
        # trans: radial arc
        ########################################################

        angles = []

        for x1, y1, angle1, quality1, chr1 in new_points.values():

            # reduce clutter by not writing arc to the same angle
            for x2, y2, angle2, quality2, chr2 in self.mPreviousPoints.values():

                for a in angles:
                    if a - self.mAngleResolution < angle2 < a + self.mAngleResolution:
                        break
                else:
                    angles.append(angle2)

                    d = SVGdraw.pathdata(x1, y1)

                    if chr1 == chr2:
                        d.relellarc(
                            self.mRadius, self.mRadius, 0, 0, 1, x2 - x1, y2 - y1)
                        link_width = link_rad_width
                    else:
                        d.relellarc(
                            self.mRadius * 2, self.mRadius * 2, 0, 0, 0, x2 - x1, y2 - y1)
                        link_width = link_arc_width

                    e = SVGdraw.path(d,
                                     fill="none",
                                     stroke="rgb(%i,%i,%i)" % link_colour,
                                     stroke_width=link_width)

                    self.addWheelElement(e, self.mPlaneLinks)

        # plot lines between new points
        new_genes = new_points.keys()

        for g1 in range(len(new_genes) - 1):

            x1, y1, angle1, quality1, chr1 = new_points[new_genes[g1]]

            for g2 in range(g1 + 1, len(new_genes)):

                x2, y2, angle2, quality2, chr2 = new_points[new_genes[g2]]

                for a in angles:
                    if a - self.mAngleResolution < angle2 < a + self.mAngleResolution:
                        break
                else:
                    angles.append(angle2)

                    d = SVGdraw.pathdata(x1, y1)

                    if chr1 == chr2:
                        d.relellarc(
                            self.mRadius, self.mRadius, 0, 0, 1, x2 - x1, y2 - y1)
                        link_width = link_rad_width
                    else:
                        d.relellarc(
                            self.mRadius * 2, self.mRadius * 2, 0, 0, 0, x2 - x1, y2 - y1)
                        link_width = link_arc_width

                    e = SVGdraw.path(d,
                                     fill="none",
                                     stroke="rgb(%i,%i,%i)" % link_colour,
                                     stroke_width=link_width)

                    self.addWheelElement(e, self.mPlaneLinks)

        # add new points to old points
        for k, v in new_points.items():
            self.mPreviousPoints[k] = v