def _test_interaction(self, ngenes: int, test: str, constrained: bool,
spline_basis: str):
n_timepoints = 5
sim = Simulator(num_observations=n_timepoints * 200,
num_features=ngenes)
sim.generate_sample_description(num_batches=0, num_conditions=0)
sim.generate_params()
sim.generate_data()
random_sample_description = pd.DataFrame({
"continuous":
np.asarray(np.random.randint(0, n_timepoints, size=sim.nobs),
dtype=float)
})
random_sample_description["condition"] = [
str(np.random.randint(0, 2))
for x in random_sample_description["continuous"]
]
random_sample_description["batch"] = [
x + str(np.random.randint(0, 3))
for x in random_sample_description["condition"]
]
random_sample_description["size_factors"] = np.random.uniform(
0.9, 1.1, sim.nobs) # TODO put into simulation.
det = self._fit_continuous_interaction(
sim=sim,
sample_description=random_sample_description,
test=test,
constrained=constrained,
spline_basis=spline_basis,
)
return det
def test(self):
"""
Check that factors that are numeric receive the correct number of coefficients.
:return:
"""
logging.getLogger("tensorflow").setLevel(logging.ERROR)
logging.getLogger("batchglm").setLevel(logging.WARNING)
logging.getLogger("diffxpy").setLevel(logging.WARNING)
sim = Simulator(num_observations=2000, num_features=2)
sim.generate_sample_description(num_batches=0, num_conditions=2)
sim.generate_params()
sim.generate_data()
sample_description = sim.sample_description
sample_description["numeric1"] = np.random.random(size=sim.nobs)
sample_description["numeric2"] = np.random.random(size=sim.nobs)
test = de.test.wald(
data=sim.input_data,
sample_description=sample_description,
formula_loc="~ 1 + condition + numeric1 + numeric2",
formula_scale="~ 1",
factor_loc_totest="condition",
as_numeric=["numeric1", "numeric2"],
training_strategy="DEFAULT")
# Check that number of coefficients is correct.
assert test.model_estim.a_var.shape[0] == 4
return True
def _prepare_data(self, n_cells: int, n_genes: int, noise_model: str):
"""
:param n_cells: Number of cells to simulate (number of observations per test).
:param n_genes: Number of genes to simulate (number of tests).
:param noise_model: Noise model to use for data fitting.
"""
if noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
rand_fn_loc = lambda shape: np.random.uniform(5, 10, shape)
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
elif noise_model == "norm":
from batchglm.api.models import Simulator
rand_fn_loc = lambda shape: np.random.uniform(500, 1000, shape)
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
else:
raise ValueError("noise model %s not recognized" % noise_model)
num_non_de = n_genes // 2
sim = Simulator(num_observations=n_cells, num_features=n_genes)
sim.generate_sample_description(num_batches=0, num_conditions=2)
sim.generate_params(rand_fn_loc=rand_fn_loc,
rand_fn_scale=rand_fn_scale)
sim.a_var[1, :num_non_de] = 0
sim.b_var[1, :num_non_de] = 0
self.isDE = np.arange(n_genes) >= num_non_de
sim.generate_data()
return sim
def _test_single_full_rank(self):
"""
Test if de.wald() generates a uniform p-value distribution
if it is given data simulated based on the null model. Returns the p-value
of the two-side Kolmgorov-Smirnov test for equality of the observed
p-value distribution and a uniform distribution.
:param n_cells: Number of cells to simulate (number of observations per test).
:param n_genes: Number of genes to simulate (number of tests).
:param noise_model: Noise model to use for data fitting.
"""
if self.noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
elif self.noise_model == "norm":
from batchglm.api.models import Simulator
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
else:
raise ValueError("noise model %s not recognized" % self.noise_model)
sim = Simulator(num_observations=200, num_features=2)
sim.generate_sample_description(num_batches=0, num_conditions=0)
sim.generate_params(rand_fn_scale=rand_fn_scale)
sim.generate_data()
random_sample_description = pd.DataFrame({
"condition": [str(x) for x in np.random.randint(2, size=sim.nobs)]
})
try:
random_sample_description["batch"] = random_sample_description["condition"]
_ = de.test.wald(
data=sim.input_data,
sample_description=random_sample_description,
factor_loc_totest="condition",
formula_loc="~ 1 + condition + batch",
noise_model=self.noise_model
)
except ValueError as error:
logging.getLogger("diffxpy").info(error)
else:
raise ValueError("rank error was erroneously not thrown on under-determined unconstrained system")
try:
random_sample_description["batch"] = [
x + str(np.random.randint(0, 2)) for x in random_sample_description["condition"].values
]
_ = de.test.wald(
data=sim.input_data,
sample_description=random_sample_description,
factor_loc_totest="condition",
formula_loc="~ 1 + condition + batch",
constraints_loc={"batch": "condition"},
noise_model=self.noise_model
)
except ValueError as error:
raise ValueError("rank error was erroneously thrown on defined constrained system")
class TestSimulationGlmAll:
sim: _SimulatorGLM
input_data: InputDataGLM
noise_model: str
def eval_simulation_mean(self):
if self.noise_model is None:
raise ValueError("noise_model is None")
else:
if self.noise_model == "nb":
threshold_dev = 1e-2
threshold_std = 1e-1
elif self.noise_model == "norm":
threshold_dev = 1e-2
threshold_std = 1e-1
elif self.noise_model == "beta":
threshold_dev = 1e-2
threshold_std = 1e-1
else:
raise ValueError("noise_model not recognized")
means_sim = self.sim.a_var[0, :]
means_obs = self.sim.link_loc(np.mean(self.sim.input_data.x, axis=0))
mean_dev = np.mean(means_sim - means_obs)
std_dev = np.std(means_sim - means_obs)
logging.getLogger("batchglm").info("mean_dev_a %f" % mean_dev)
logging.getLogger("batchglm").info("std_dev_a %f" % std_dev)
if np.abs(mean_dev) < threshold_dev and \
std_dev < threshold_std:
return True
else:
return False
def _test_all_moments(self):
if self.noise_model is None:
raise ValueError("noise_model is None")
else:
if self.noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
elif self.noise_model == "norm":
from batchglm.api.models import Simulator
elif self.noise_model == "beta":
from batchglm.api.models.tf1.glm_beta import Simulator
else:
raise ValueError("noise_model not recognized")
self.sim = Simulator(num_observations=100000, num_features=10)
self.sim.generate_sample_description(num_batches=1, num_conditions=1)
self.sim.generate_params()
self.sim.generate_data()
success = self.eval_simulation_mean()
assert success, "mean of simulation was inaccurate"
return True
def _test_wald_de(
self,
constrained: bool,
spline_basis: str,
ngenes: int
):
if self.noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
rand_fn_loc = lambda shape: np.random.uniform(2, 5, shape)
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
elif self.noise_model == "norm":
from batchglm.api.models import Simulator
rand_fn_loc = lambda shape: np.random.uniform(500, 1000, shape)
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
else:
raise ValueError("noise model %s not recognized" % self.noise_model)
n_timepoints = 7
sim = Simulator(num_observations=n_timepoints*200, num_features=ngenes)
sim.generate_sample_description(
num_batches=0,
num_conditions=n_timepoints
)
sim.generate_params(
rand_fn_loc=rand_fn_loc,
rand_fn_scale=rand_fn_scale
)
num_non_de = round(ngenes / 2)
sim.a_var[1:, :num_non_de] = 0 # Set all condition effects of non DE genes to zero.
sim.b_var[1:, :] = 0 # Use constant dispersion across all conditions.
self.isDE = np.arange(ngenes) >= num_non_de
sim.generate_data()
random_sample_description = sim.sample_description
random_sample_description["continuous"] = [int(x) for x in random_sample_description["condition"]]
random_sample_description["batch"] = [
str(int(x)) + str(np.random.randint(0, 3))
for x in random_sample_description["continuous"]
]
test = de.test.continuous_1d(
data=sim.input_data,
sample_description=random_sample_description,
gene_names=["gene" + str(i) for i in range(sim.input_data.num_features)],
formula_loc="~ 1 + continuous + batch" if constrained else "~ 1 + continuous",
formula_scale="~ 1",
factor_loc_totest="continuous",
continuous="continuous",
constraints_loc={"batch": "continuous"} if constrained else None,
df=5,
spline_basis=spline_basis,
test="wald",
quick_scale=True,
noise_model=self.noise_model
)
self._eval(sim=sim, test=test)
def _test_null_distribution_wald(self, n_cells: int, n_genes: int,
noise_model: str):
"""
Test if de.wald() generates a uniform p-value distribution
if it is given data simulated based on the null model. Returns the p-value
of the two-side Kolmgorov-Smirnov test for equality of the observed
p-value distribution and a uniform distribution.
:param n_cells: Number of cells to simulate (number of observations per test).
:param n_genes: Number of genes to simulate (number of tests).
:param noise_model: Noise model to use for data fitting.
"""
if noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
elif noise_model == "norm":
from batchglm.api.models import Simulator
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
else:
raise ValueError("noise model %s not recognized" % noise_model)
sim = Simulator(num_observations=n_cells, num_features=n_genes)
sim.generate_sample_description(num_batches=0, num_conditions=0)
sim.generate_params(rand_fn_scale=rand_fn_scale)
sim.generate_data()
random_sample_description = pd.DataFrame({
"condition":
np.random.randint(2, size=sim.nobs),
"batch":
np.random.randint(2, size=sim.nobs)
})
random_sf = np.random.uniform(0.5, 1.5, sim.nobs)
test = de.test.wald(data=sim.input_data,
sample_description=random_sample_description,
factor_loc_totest="condition",
formula_loc="~ 1 + condition + batch",
size_factors=random_sf,
batch_size=500,
noise_model=noise_model,
training_strategy="DEFAULT",
dtype="float64")
_ = test.summary()
# Compare p-value distribution under null model against uniform distribution.
pval_h0 = stats.kstest(test.pval, 'uniform').pvalue
logging.getLogger("diffxpy").info(
'KS-test pvalue for null model match of wald(): %f' % pval_h0)
assert pval_h0 > 0.05, ("KS-Test failed: pval_h0=%f is <= 0.05!" %
np.round(pval_h0, 5))
return True
def _prepare_data(self, n_cells: int = 2000, n_genes: int = 100):
"""
:param n_cells: Number of cells to simulate (number of observations per test).
:param n_genes: Number of genes to simulate (number of tests).
"""
sim = Simulator(num_observations=n_cells, num_features=n_genes)
sim.generate_sample_description(num_batches=0, num_conditions=2)
sim.generate_params()
sim.generate_data()
return sim
def _test_model_fit_partition(
self,
n_cells: int,
n_genes: int,
noise_model: str
):
"""
Test if de.wald() generates a uniform p-value distribution
if it is given data simulated based on the null model. Returns the p-value
of the two-side Kolmgorov-Smirnov test for equality of the observed
p-value distribution and a uniform distribution.
:param n_cells: Number of cells to simulate (number of observations per test).
:param n_genes: Number of genes to simulate (number of tests).
:param noise_model: Noise model to use for data fitting.
"""
if noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
elif noise_model == "norm":
from batchglm.api.models.tf1.glm_norm import Simulator
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
else:
raise ValueError("noise model %s not recognized" % noise_model)
sim = Simulator(num_observations=n_cells, num_features=n_genes)
sim.generate_sample_description(num_batches=0, num_conditions=0)
sim.generate_params(rand_fn_scale=rand_fn_scale)
sim.generate_data()
random_sample_description = pd.DataFrame({
"condition": np.random.randint(2, size=sim.nobs),
"batch": np.random.randint(2, size=sim.nobs)
})
partition = de.fit.partition(
data=sim.input_data,
sample_description=random_sample_description,
parts="condition"
)
estim = partition.model(
formula_loc="~ 1 + batch",
noise_model=noise_model
)
return True
def _prepate_data(self, n_cells: int, n_genes: int, n_groups: int):
if self.noise_model == "nb":
from batchglm.api.models.tf1.glm_nb import Simulator
rand_fn_loc = lambda shape: np.random.uniform(0.1, 1, shape)
rand_fn_scale = lambda shape: np.random.uniform(0.5, 1, shape)
elif self.noise_model == "norm" or self.noise_model is None:
from batchglm.api.models import Simulator
rand_fn_loc = lambda shape: np.random.uniform(500, 1000, shape)
rand_fn_scale = lambda shape: np.random.uniform(1, 2, shape)
else:
raise ValueError("noise model %s not recognized" %
self.noise_model)
sim = Simulator(num_observations=n_cells, num_features=n_genes)
sim.generate_sample_description(num_batches=0, num_conditions=0)
sim.generate_params(rand_fn_loc=rand_fn_loc,
rand_fn_scale=rand_fn_scale)
sim.generate_data()
random_sample_description = pd.DataFrame({
"condition":
[str(x) for x in np.random.randint(n_groups, size=sim.nobs)]
})
return sim, random_sample_description