示例#1
0
    #---------------------------------------------------------------------------------------

    logger.info('***\t*\t--> Started %s method execution - fluidics_utils.py' %
                sys.arg[2])

    if method is 'clean_V1_to_syringe':
        biochem.clean_V1_to_syringe()

    elif method is 'clean_V2_to_syringe':
        biochem.clean_V2_to_syringe()

    elif method is 'clean_flowcell_and_syringe':
        biochem.clean_flowcell_and_syringe()

    elif method is 'cycle_ligation':
        biochem.cycle_ligation()

    elif method is 'draw_air_to_mixer':
        print "\n***\t*\t--> Please, enter air gap size [integer]: ",
        gap_size = int(sys.stdin.readline().strip(
        ))  # use stdin explicitly and remove trailing new-line character
        biochem.draw_air_to_mixer(gap_size)

    elif method is 'draw_air_to_syringe':
        biochem.draw_air_to_syringe()

    elif method is 'draw_air_to_valve':
        print "\n***\t*\t--> Please, enter valve name air to be drawn (V1, V2, V3, V5) [string]: ",
        valve = str(sys.stdin.readline().strip(
        ))  # use stdin explicitly and remove trailing new-line character
        biochem.draw_air_to_valve(valve)
示例#2
0
    biochem = Biochem('WL1', int(sys.argv[1]))  
    method = sys.argv[2]
    log.info("---\t-\t--> Started %s method execution - biochem_utils.py" % \
                (method))



    # usage: biochem_utils.py fcnum cycle_ligation cyclename
    # cyclename must be a valid cyclename that has a nonamer/hyb
    # valve/port mapping in sequencing.cfg
    # cyclename can be either 3 or 4 chars in length
    if method == 'cycle_ligation':
        biochem.flowcell = int(sys.argv[1])
        biochem.cycle_name = sys.argv[3]
        biochem.cycle = biochem.cycle_name[0:3]
        biochem.cycle_ligation()


    # usage: biochem_utils.py fcnum flush_flowcell
    elif method == 'flush_flowcell':
        reagent = "Wash 1"
        if reagent == 'Wash 1':
            port = 9
        elif reagent == 'dH2O':
            port = 7

        biochem.flush_flowcell(9)
        #biochem.rinse(int(sys.argv[1]))

    # usage: biochem_utils.py fcnum draw_into_flowcell
    elif method == 'draw_into_flowcell':