# FINDS THE TISSUE AREA
            img2 = imp.duplicate()
            channels2=ChannelSplitter.split(img2);
            redimg2=channels2[0];
            IJ.run(redimg2, "8-bit", "");
            IJ.setAutoThreshold(redimg2, "Default dark");
            IJ.setThreshold(redimg2,20, 254);
            IJ.run(redimg2, "Convert to Mask", "");
            redimg2.show()
            time.sleep(1)
            rt2 = ResultsTable()
            ta=Analyzer(redimg2,Measurements.AREA|Measurements.LIMIT,rt2)
            ta.measure();
            double=rt2.getColumnAsDoubles(rt2.getColumnIndex("Area"))
            summary["Tissue-area"] =double[0];
            redimg2.changes = False
            redimg2.close()
            	

#    PARTICLE ANALYSIS ETC..
                
            channels = ChannelSplitter.split(imp);
            
            
            
            for i, channel in enumerate(channels):
                IJ.setAutoThreshold(channel,"Default");
                summary[color[i] + "-intensity"] = "NA"
                summary[color[i] + "-ROI-count"] = "NA"