def main(tableName, showPlot):
image = IJ.getImage();
roi = image.getRoi()
if not roi:
center = image.getWidth() / 2, image.getHeight() / 2
else:
center = roi.getXBase(), roi.getYBase();
table = ResultsTable.getResultsTable(tableName)
vectors = getVectorsFromTable(table, center)
radialVelocity = calculateRadialVelocityPerTime(vectors, center)
radialVelocityAndDistanceByTrack(table, center)
stats = Tools.getStatistics(radialVelocity)
median = calculateMedian(radialVelocity)
rt = ResultsTable.getResultsTable(TABLE_NAME)
if not rt:
rt = ResultsTable()
row = rt.getCounter()
rt.setValue("label", row, tableName)
rt.setValue("x", row, center[0])
rt.setValue("y", row, center[1])
rt.setValue("mean", row, stats.mean)
rt.setValue("stdDev", row, stats.stdDev)
rt.setValue("min", row, stats.min)
rt.setValue("median", row, median)
rt.setValue("max", row, stats.max)
rt.show(TABLE_NAME)
if showPlot:
plot(radialVelocity, center)
def analyze(imp, min_area):
MAXSIZE = 1000000000000
MINCIRCULARITY = 0.0
MAXCIRCULARITY = 1.
options = PA.SHOW_MASKS
temp_results = ResultsTable()
p = PA(options, PA.AREA + PA.MEAN, temp_results, min_area, MAXSIZE, MINCIRCULARITY, MAXCIRCULARITY)
p.setHideOutputImage(True)
p.analyze(imp)
if temp_results.getCounter() == 0:
areas = []
signals = []
else:
areas = list(temp_results.getColumn(0))
signals = list(temp_results.getColumn(1))
count = len(areas)
area = sum(areas)
total = 0
if area > 0:
total = sum([a*s for a,s in zip(areas, signals)]) / area
return p.getOutputImage(), count, area, total
def main(tableName, showPlot):
image = IJ.getImage();
roi = image.getRoi()
if not roi:
center = image.getWidth() / 2, image.getHeight() / 2
else:
center = roi.getXBase(), roi.getYBase();
table = ResultsTable.getResultsTable(tableName)
rma = RadialMovementAnalyzer(table, center)
radialDistances = rma.getDeltaRadialDistancePerTrack()
distances = rma.getDistances()
frames = rma.getFrames()
travelledDistances = rma.getTravelledDistances()
TABLE_NAME = "Distance from " + str(center)
rt = ResultsTable.getResultsTable(TABLE_NAME)
if not rt:
rt = ResultsTable()
for index, dist in enumerate(radialDistances):
row = rt.getCounter()
rt.setValue("label", row, tableName)
rt.setValue("track ID", row, rma.trackIDs[index])
rt.setValue("total augmentation of distance from center", row, dist)
rt.setValue("distance start to end", row, distances[index])
rt.setValue("travelled distance", row, travelledDistances[index])
rt.setValue("nr. of frames", row, frames[index])
if not distances[index] == 0:
rt.setValue("total augmentation / distance start to end", row, dist / distances[index])
else:
rt.setValue("total augmentation / distance start to end", row, float("nan"))
if not travelledDistances[index] ==0:
rt.setValue("total augmentation / travelled distance", row, dist / travelledDistances[index])
else:
rt.setValue("total augmentation / travelled distance", row, float("nan"))
rt.setValue("mean speed", row, travelledDistances[index] / frames[index])
rt.setValue("mean outward speed", row, dist / frames[index])
rt.show(TABLE_NAME)
if showPlot:
plot(distances, radialDistances, center)
class MandersPlugin(ImageListener, WindowAdapter):
def __init__(self):
self.imp = None
self.preview = None
self.createMainWindow()
self.cells = None
self.files = []
self.results = ResultsTable()
ImagePlus.addImageListener(self)
self.selectInputDir()
self.selectOutputDir()
self.pairs = []
self.methods = []
self.processNextFile()
def selectInputDir(self):
inputDialog = DirectoryChooser("Please select a directory contaning your images")
inputDir = inputDialog.getDirectory()
for imageFile in os.listdir(inputDir):
self.files.append(inputDir + imageFile)
def selectOutputDir(self):
outputDialog = DirectoryChooser("Please select a directory to save your results")
self.outputDir = outputDialog.getDirectory()
def closeImage(self):
if self.imp is not None:
self.imp.close()
self.imp = None
if self.preview is not None:
self.preview.close()
self.preview = None
def openImage(self, imageFile):
try:
images = BF.openImagePlus(imageFile)
self.imp = images[0]
except UnknownFormatException:
return None
if self.imp.getNChannels() < 2:
IJ.error("Bad image format", "Image must contain at lease 2 channels!")
return None
if not self.pairs or \
not self.methods:
self.getOptionsDialog(self.imp)
title = self.imp.title
self.imp.title = title[:title.rfind('.')]
return self.imp
def getOptionsDialog(self, imp):
thr_methods = ["None", "Default", "Huang", "Intermodes", "IsoData", "Li", "MaxEntropy","Mean", "MinError(I)", "Minimum", "Moments", "Otsu", "Percentile", "RenyiEntropy", "Shanbhag" , "Triangle", "Yen"]
gd = GenericDialog("Please select channels to collocalize")
for i in range(1, imp.getNChannels() + 1):
gd.addChoice("Threshold method for channel %i" % i, thr_methods, "None")
gd.showDialog()
if gd.wasCanceled():
self.exit()
channels = []
for i in range(1, imp.getNChannels() + 1):
method = gd.getNextChoice()
self.methods.append(method)
if method != "None":
channels.append(i)
for x in channels:
for y in channels:
if x < y:
self.pairs.append((x, y))
def processNextFile(self):
if self.files:
imageFile = self.files.pop(0)
return self.processFile(imageFile)
else:
return False
def processFile(self, imageFile):
imp = self.openImage(imageFile)
if imp is not None:
cell = Cell(imp.NSlices, 1)
self.cells = DelegateListModel([])
self.cells.append(cell)
self.showMainWindow(self.cells)
if self.checkbox3D.isSelected():
self.displayImage(imp)
else:
self.displayImage(imp, False)
self.preview = self.previewImage(imp)
self.displayImage(self.preview)
return True
else:
return self.processNextFile()
def displayImage(self, imp, show = True):
imp.setDisplayMode(IJ.COMPOSITE)
enhancer = ContrastEnhancer()
enhancer.setUseStackHistogram(True)
splitter = ChannelSplitter()
for c in range(1, imp.getNChannels() + 1):
imp.c = c
enhancer.stretchHistogram(imp, 0.35)
if show:
imp.show()
def previewImage(self, imp):
roi = imp.getRoi()
splitter = ChannelSplitter()
channels = []
for c in range(1, imp.getNChannels() + 1):
channel = ImagePlus("Channel %i" % c, splitter.getChannel(imp, c))
projector = ZProjector(channel)
projector.setMethod(ZProjector.MAX_METHOD)
projector.doProjection()
channels.append(projector.getProjection())
image = RGBStackMerge.mergeChannels(channels, False)
image.title = imp.title + " MAX Intensity"
image.luts = imp.luts
imp.setRoi(roi)
return image
def getCroppedChannels(self, imp, cell):
splitter = ChannelSplitter()
imp.setRoi(None)
if cell.mode3D:
cropRoi = cell.getCropRoi()
else:
cropRoi = cell.roi
if cropRoi is None:
return None
crop = cropRoi.getBounds()
channels = []
for c in range(1, imp.getNChannels() + 1):
slices = ImageStack(crop.width, crop.height)
channel = splitter.getChannel(imp, c)
for z in range(1, channel.getSize() + 1):
zslice = channel.getProcessor(z)
zslice.setRoi(cropRoi)
nslice = zslice.crop()
if cell.mode3D:
oroi = cell.slices[z - 1].roi
else:
oroi = cell.roi
if oroi is not None:
roi = oroi.clone()
bounds = roi.getBounds()
roi.setLocation(bounds.x - crop.x, bounds.y - crop.y)
nslice.setColor(Color.black)
nslice.fillOutside(roi)
slices.addSlice(nslice)
channels.append(ImagePlus("Channel %i" % c, slices))
return channels
def getThreshold(self, imp, method):
thresholder = Auto_Threshold()
duplicator = Duplicator()
tmp = duplicator.run(imp)
return thresholder.exec(tmp, method, False, False, True, False, False, True)
def getContainer(self, impA, impB):
imgA = ImagePlusAdapter.wrap(impA)
imgB = ImagePlusAdapter.wrap(impB)
return DataContainer(imgA, imgB, 1, 1, "imageA", "imageB")
def getManders(self, imp, cell):
### Crop channels according to cell mask
channels = self.getCroppedChannels(imp, cell)
if channels is None:
return None
### Calculate channel thresholds
thrs = []
thrimps = []
for c, method in enumerate(self.methods):
if method != "None":
thr, thrimp = self.getThreshold(channels[c], method)
else:
thr, thrimp = None, None
thrs.append(thr)
thrimps.append(thrimp)
### Calculate manders colocalization
manders = MandersColocalization()
raws = []
thrds = []
for chA, chB in self.pairs:
container = self.getContainer(channels[chA - 1], channels[chB - 1])
img1 = container.getSourceImage1()
img2 = container.getSourceImage2()
mask = container.getMask()
cursor = TwinCursor(img1.randomAccess(), img2.randomAccess(), Views.iterable(mask).localizingCursor())
rtype = img1.randomAccess().get().createVariable()
raw = manders.calculateMandersCorrelation(cursor, rtype)
rthr1 = rtype.copy()
rthr2 = rtype.copy()
rthr1.set(thrs[chA - 1])
rthr2.set(thrs[chB - 1])
cursor.reset()
thrd = manders.calculateMandersCorrelation(cursor, rthr1, rthr2, ThresholdMode.Above)
raws.append(raw)
thrds.append(thrd)
return (channels, thrimps, thrs, raws, thrds)
def saveMultichannelImage(self, title, channels, luts):
tmp = RGBStackMerge.mergeChannels(channels, False)
tmp.luts = luts
saver = FileSaver(tmp)
saver.saveAsTiffStack(self.outputDir + title + ".tif")
tmp.close()
def createMainWindow(self):
self.frame = JFrame('Select cells and ROIs',
defaultCloseOperation = JFrame.DISPOSE_ON_CLOSE
)
self.frame.setLayout(GridBagLayout())
self.frame.addWindowListener(self)
self.frame.add(JLabel("Cells"),
GridBagConstraints(0, 0, 1, 1, 0, 0,
GridBagConstraints.CENTER, GridBagConstraints.NONE,
Insets(5, 2, 2, 0), 0, 0
))
self.cellList = JList(DelegateListModel([]),
selectionMode = ListSelectionModel.SINGLE_SELECTION,
cellRenderer = MyRenderer(),
selectedIndex = 0,
valueChanged = self.selectCell
)
self.frame.add(JScrollPane(self.cellList),
GridBagConstraints(0, 1, 1, 5, .5, 1,
GridBagConstraints.CENTER, GridBagConstraints.BOTH,
Insets(0, 2, 2, 0), 0, 0
))
self.frame.add(JButton('Add cell', actionPerformed = self.addCell),
GridBagConstraints(1, 2, 1, 2, 0, .25,
GridBagConstraints.CENTER, GridBagConstraints.NONE,
Insets(0, 0, 0, 0), 0, 0
))
self.frame.add(JButton('Remove cell', actionPerformed = self.removeCell),
GridBagConstraints(1, 4, 1, 2, 0, .25,
GridBagConstraints.CENTER, GridBagConstraints.NONE,
Insets(0, 5, 0, 5), 0, 0
))
self.frame.add(JLabel("Slices"),
GridBagConstraints(0, 6, 1, 1, 0, 0,
GridBagConstraints.CENTER, GridBagConstraints.NONE,
Insets(5, 2, 2, 0), 0, 0
))
self.sliceList = JList(DelegateListModel([]),
selectionMode = ListSelectionModel.SINGLE_SELECTION,
cellRenderer = MyRenderer(),
selectedIndex = 0,
valueChanged = self.selectSlice
)
self.frame.add(JScrollPane(self.sliceList),
GridBagConstraints(0, 7, 1, 5, .5, 1,
GridBagConstraints.CENTER, GridBagConstraints.BOTH,
Insets(0, 2, 2, 0), 0, 0
))
self.frame.add(JButton('Update ROI', actionPerformed = self.updateSlice),
GridBagConstraints(1, 8, 1, 2, 0, .25,
GridBagConstraints.CENTER, GridBagConstraints.NONE,
Insets(0, 0, 0, 0), 0, 0
))
self.frame.add(JButton('Done', actionPerformed = self.doneSelecting),
GridBagConstraints(1, 10, 1, 2, 0, .25,
GridBagConstraints.CENTER, GridBagConstraints.NONE,
Insets(0, 0, 0, 0), 0, 0
))
self.checkbox3D = JCheckBox('3D selection mode', True, actionPerformed=self.toggle3D)
self.frame.add(self.checkbox3D,
GridBagConstraints(0, 13, 2, 1, 0, 1,
GridBagConstraints.WEST, GridBagConstraints.NONE,
Insets(0, 0, 0, 0), 0, 0
))
def showMainWindow(self, cells = None):
if cells is not None:
self.cellList.model = cells
if cells:
self.cellList.selectedIndex = 0
self.frame.pack()
self.frame.visible = True
def hideMainWindow(self):
self.frame.visible = False
def closeMainWindow(self):
self.frame.dispose()
def toggle3D(self, event):
mode3D = self.checkbox3D.isSelected()
if mode3D:
self.sliceList.enabled = True
if self.imp is not None:
self.imp.show()
if self.preview is not None:
self.preview.hide()
else:
self.sliceList.enabled = False
if self.preview is None:
self.preview = self.previewImage(self.imp)
self.displayImage(self.preview)
else:
self.preview.show()
if self.imp is not None:
self.imp.hide()
selectedCell = self.cellList.selectedIndex
if selectedCell >= 0:
cell = self.cells[selectedCell]
self.sliceList.model = cell.slices
self.sliceList.selectedIndex = 0
def addCell(self, event):
size = len(self.cells)
if (size > 0):
last = self.cells[size - 1]
n = last.n + 1
else:
n = 1
self.cells.append(Cell(self.imp.NSlices, n))
self.cellList.selectedIndex = size
def removeCell(self, event):
selected = self.cellList.selectedIndex
if selected >= 0:
self.cells.remove(self.cells[selected])
if (selected >= 1):
self.cellList.selectedIndex = selected - 1
else:
self.cellList.selectedIndex = 0
def selectCell(self, event):
selected = self.cellList.selectedIndex
if selected >= 0:
cell = self.cells[selected]
self.sliceList.model = cell.slices
self.sliceList.selectedIndex = 0
else:
self.sliceList.model = DelegateListModel([])
if self.preview is not None:
self.preview.setRoi(cell.roi)
def selectSlice(self, event):
selectedCell = self.cellList.selectedIndex
selectedSlice = self.sliceList.selectedIndex
if selectedCell >= 0 and selectedSlice >= 0:
cell = self.cells[selectedCell]
image = self.imp
mode3D = self.checkbox3D.isSelected()
if image is not None and cell is not None and mode3D:
roi = cell.slices[selectedSlice].roi
if (image.z - 1 != selectedSlice):
image.z = selectedSlice + 1
image.setRoi(roi, True)
if self.preview is not None and not mode3D:
self.preview.setRoi(cell.roi, True)
def updateSlice(self, event):
if self.checkbox3D.isSelected():
self.updateSlice3D(self.imp)
else:
self.updateSlice2D(self.preview)
def updateSlice3D(self, imp):
selectedCell = self.cellList.selectedIndex
selectedSlice = self.sliceList.selectedIndex
if selectedCell >= 0 and selectedSlice >= 0 and imp is not None:
cell = self.cells[selectedCell]
impRoi = imp.getRoi()
if cell is not None and impRoi is not None:
index = selectedSlice + 1
roi = ShapeRoi(impRoi, position = index)
cell.mode3D = True
cell.name = "Cell %i (3D)" % cell.n
cell.slices[selectedSlice].roi = roi
if (index + 1 <= len(cell.slices)):
imp.z = index + 1
self.cellList.repaint(self.cellList.getCellBounds(selectedCell, selectedCell))
self.sliceList.repaint(self.sliceList.getCellBounds(selectedSlice, selectedSlice))
def updateSlice2D(self, imp):
selectedCell = self.cellList.selectedIndex
if selectedCell >= 0 and imp is not None:
cell = self.cells[selectedCell]
impRoi = imp.getRoi()
if cell is not None and impRoi is not None:
roi = ShapeRoi(impRoi, position = 1)
cell.mode3D = False
cell.name = "Cell %i (2D)" % cell.n
cell.roi = roi
self.cellList.repaint(self.cellList.getCellBounds(selectedCell, selectedCell))
def imageOpened(self, imp):
pass
def imageClosed(self, imp):
pass
def imageUpdated(self, imp):
if self.checkbox3D.isSelected():
if imp is not None:
selectedCell = self.cellList.selectedIndex
selectedSlice = imp.z - 1
if imp == self.imp and selectedSlice != self.sliceList.selectedIndex:
self.sliceList.selectedIndex = selectedSlice
def doneSelecting(self, event):
oluts = self.imp.luts
luts = []
channels = []
for c, method in enumerate(self.methods):
if method != "None":
luts.append(oluts[c])
channels.append(c)
for cell in self.cells:
manders = self.getManders(self.imp, cell)
if manders is not None:
chimps, thrimps, thrs, raws, thrds = manders
index = self.cells.index(cell) + 1
title = "Cell_%i-" % index + self.imp.title
self.saveMultichannelImage(title, chimps, oluts)
title = "Cell_%i_thrd-" % index + self.imp.title
self.saveMultichannelImage(title, thrimps, luts)
self.results.incrementCounter()
row = self.results.getCounter() - 1
for i, thr in enumerate(thrs):
if thr is not None:
self.results.setValue("Threshold %i" % (i + 1), row, int(thr))
for i, pair in enumerate(self.pairs):
self.results.setValue("%i-%i M1 raw" % pair, row, float(raws[i].m1))
self.results.setValue("%i-%i M2 raw" % pair, row, float(raws[i].m2))
self.results.setValue("%i-%i M1 thrd" % pair, row, float(thrds[i].m1))
self.results.setValue("%i-%i M2 thrd" % pair, row, float(thrds[i].m2))
self.closeImage()
if not self.processNextFile():
print "All done - happy analysis!"
self.results.show("Manders collocalization results")
self.exit()
def windowClosing(self, e):
print "Closing plugin - BYE!!!"
self.exit()
def exit(self):
ImagePlus.removeImageListener(self)
self.closeImage()
self.closeMainWindow()
# + PA.SHOW_RESULTS \
rt = ResultsTable()
p = PA(options, PA.AREA + PA.STACK_POSITION, rt, MINSIZE, MAXSIZE)
p.setHideOutputImage(True)
# Morphological dilate
binner.setup('dilate', None)
clusters = 0
initialCells = 0
# dilate by 'SAMPLEITER'
for i in range(SAMPLEITER+1):
p.analyze(binimp)
cellcounts = rt.getCounter()
if i == 0:
initialCells = cellcounts
#IJ.log("iter:" + str(i) + " -- cell counts: " + str(cellcounts))
if i == SAMPLEITER:
clusters = cellcounts
binner.run(binimp.getProcessor())
rt.reset()
#binimp.show()
#binorg.show()
IJ.log("==== " + imp3.getTitle() + " =====")
IJ.log("Number of Nucleus : " + str(initialCells))
IJ.log("Clusters at dilation " + str(SAMPLEITER) + ": " + str(clusters))
IJ.log("Clusters/Nucleus " + str(float(clusters)/float(initialCells)))
thr1, thrimp1 = calculateThreshold(imp1, roi, methods[0])
thr2, thrimp2 = calculateThreshold(imp2, roi, methods[1])
cursor = TwinCursor(img1.randomAccess(), img2.randomAccess(), Views.iterable(mask).localizingCursor())
rtype = img1.randomAccess().get().createVariable()
raw = manders.calculateMandersCorrelation(cursor, rtype)
rthr1 = rtype.copy()
rthr2 = rtype.copy()
rthr1.set(thr1)
rthr2.set(thr2)
cursor.reset()
thrd = manders.calculateMandersCorrelation(cursor, rthr1, rthr2, ThresholdMode.Above)
print "Results are: %f %f %f %f" % (raw.m1, raw.m2, thrd.m1, thrd.m2)
results.incrementCounter()
rowno = results.getCounter() - 1
results.setValue("Cell", rowno, int(rowno))
results.setValue("Threshold 1", rowno, int(thr1))
results.setValue("Threshold 2", rowno, int(thr2))
results.setValue("M1 raw", rowno, float(raw.m1))
results.setValue("M2 raw", rowno, float(raw.m2))
results.setValue("M1 thrd", rowno, float(thrd.m1))
results.setValue("M2 thrd", rowno, float(thrd.m2))
thrimp = RGBStackMerge.mergeChannels([thrimp1, thrimp2], False)
saver = FileSaver(thrimp)
saver.saveAsTiffStack(outputDir + "Cell_%i-" % results.getCounter() + title + ".tif")
thrimp.close()
results.show("Colocalization results")
def main():
rt = RT.open2(table_file.getAbsolutePath())
if not rt: return
log(" --- --- --- ")
log("Loaded %s" % table_file.getAbsolutePath())
log("Loading column lists...")
# Get column indices from imported file
headings = getColumnHeadings(rt)
id_col = getColumnIndex(headings, "TID")
t_col = getColumnIndex(headings, "t [")
d2p_col = getColumnIndex(headings, "D2P [")
angle_col = getColumnIndex(headings, u'\u03B1 [deg]')
delta_col = getColumnIndex(headings, u'\u0394\u03B1 [deg]')
if angle_col == RT.COLUMN_NOT_FOUND:
log("Failed to detect index for angle column. Re-trying...")
angle_col = getColumnIndex(headings, u'? [deg]')
if delta_col == RT.COLUMN_NOT_FOUND:
log("Failed to detect index for delta angle column. Re-trying...")
delta_col = getColumnIndex(headings, u'?? [deg]')
log("Last column index is %s" % rt.getLastColumn())
if RT.COLUMN_NOT_FOUND in (id_col, d2p_col, delta_col, angle_col):
uiservice.showDialog("Error: Some key columns were not found!",
"Invalid Table?")
return
log("Settings: BOUT_WINDOW= %s, MIN_D2P= %s, DEF_FRAME_INTERVAL= %s" %
(BOUT_WINDOW, '{0:.4f}'.format(MIN_D2P), DEF_FRAME_INTERVAL))
# Store all data on dedicated lists
track_id_rows = rt.getColumnAsDoubles(id_col)
d2p_rows = rt.getColumnAsDoubles(d2p_col)
angle_rows = rt.getColumnAsDoubles(angle_col)
delta_rows = rt.getColumnAsDoubles(delta_col)
t_rows = rt.getColumnAsDoubles(t_col)
# Assess n of data points and extract unique path ids
n_rows = len(track_id_rows)
row_indices = range(n_rows)
track_ids = set(track_id_rows)
n_tracks = len(track_ids)
log("Table has %g rows" % n_rows)
log("Table has %g tracks" % n_tracks)
log("Parsing tracks...")
for track_id in track_ids:
for row, next_row in zip(row_indices, row_indices[1:]):
if track_id_rows[row] != track_id:
continue
if not isNumber(angle_rows[row]):
rt.setValue("FLAG", row, "NA")
continue
lower_bound = max(0, row - BOUT_WINDOW + 1)
upper_bound = min(n_rows - 1, row + BOUT_WINDOW)
win_d2p = []
for _ in range(lower_bound, upper_bound):
win_d2p.append(d2p_rows[row])
if sum(win_d2p) <= MIN_D2P * len(win_d2p):
rt.setValue("FLAG", row, 0)
else:
current_angle = angle_rows[row]
next_angle = angle_rows[next_row]
current_delta = delta_rows[row]
flag = -1 if current_angle < 0 else 1
delta_change = (abs(current_delta) > 90)
same_sign = ((current_angle < 0) == (next_angle < 0))
if delta_change and not same_sign:
flag *= -1
rt.setValue("FLAG", row, flag)
if next_row == n_rows - 1:
rt.setValue("FLAG", next_row, flag)
if rt.save(table_file.getAbsolutePath()):
log("Processed table successfully saved (file overwritten)")
else:
log("Could not override input file. Displaying it...")
rt.show(table_file.name)
log("Creating onset table...")
onset_rt = RT()
onset_rt.showRowNumbers(False)
frame_int = DEF_FRAME_INTERVAL
if "table" in frame_rate_detection:
frame_int = getFrameIntervalFromTable(row_indices, track_id_rows,
t_rows)
elif "image" in frame_rate_detection:
frame_int = getFrameIntervalFromImage(image_file.getAbsolutePath())
else:
log("Using default frame rate")
for track_id in track_ids:
for prev_row, row in zip(row_indices, row_indices[1:]):
if not track_id in (track_id_rows[prev_row], track_id_rows[row]):
continue
flag = rt.getValue("FLAG", row)
if not isNumber(flag):
continue
flag = int(flag)
if flag == 0:
continue
if flag == 1 or flag == -1:
srow = onset_rt.getCounter()
onset_rt.incrementCounter()
onset_rt.setValue("TID", srow, track_id)
from_frame = int(t_rows[prev_row] / frame_int) + 1
to_frame = int(t_rows[row] / frame_int) + 1
onset_rt.setValue("First disp. [t]", srow,
"%s to %s" % (t_rows[prev_row], t_rows[row]))
onset_rt.setValue("First disp. [frames]", srow,
"%s to %s" % (from_frame, to_frame))
onset_rt.setValue("ManualTag", srow, "")
break
out_path = suffixed_path(table_file.getAbsolutePath(), "ManualTagging")
if onset_rt.save(out_path):
log("Summary table successfully saved: %s" % out_path)
else:
log("File not saved... Displaying onset table")
onset_rt.show("Onsets %s" % table_file.name)
roiStatsGfp = gfpMeasure.getStatistics()
if roiStatsGfp.area * cal.pixelWidth * cal.pixelHeight < GFPminA:
continue
bounds = gfp.getBounds()
cXgfp = int(bounds.x + (bounds.width / 2.0))
cYgfp = int(bounds.y + (bounds.height / 2.0))
if bf.contains(cXgfp, cYgfp):
gfpA += roiStatsGfp.area
gfpSum += roiStatsGfp.mean * roiStatsGfp.area
gfp.setStrokeColor(Color.MAGENTA)
gfp.setPosition(0, z, 1)
ol.add(gfp)
gfpMean = gfpSum / gfpA if gfpA > 0 else 0
row = rt.getCounter()
uni = row % 26
cha = chr(ord('A') + uni)
name = ""
while len(name) < (row + 1) / 26.0:
name += cha
label = TextRoi(name, cXbf - 20, cYbf + 20, FONT)
label.setStrokeColor(Color.GREEN)
label.setPosition(0, z, 1)
ol.add(label)
rt.setValue("Organoid", row, name)
rt.setValue("X", row, cXbf * cal.pixelWidth)
rt.setValue("Y", row, cYbf * cal.pixelHeight)
maximaip = findmaximashow.getProcessor()
maximahist = maximaip.getHistogram()
CountMethod2 = maximahist[255]
print "Using the findMaxima method with a threshold of " + str(
THRESH) + ", I found " + str(CountMethod2) + " maxima."
IJ.setRawThreshold(findmaximashow, 255, 255, "red")
IJ.run(findmaximashow, "Create Selection", "")
rm.addRoi(findmaximashow.getRoi())
rm.rename(1, "Maxima with threshold")
# ---- METHOD 3-4: API getMaxima WITH THE POINTS COUNTED OR ADDED TO THE RESULTS TABLE
maxima = mf.getMaxima(ip, 100.0, False)
CountMethod3 = maxima.npoints
print "Using the getMaxima method with no threshold , I found " + str(
CountMethod3) + " maxima."
rt = ResultsTable()
for i in range(maxima.npoints):
rt.incrementCounter()
rt.addValue("X", maxima.xpoints[i])
rt.addValue("Y", maxima.ypoints[i])
rt.show("Results")
CountMethod4 = rt.getCounter()
print "By counting points generated by getMaxima, I found " + str(
CountMethod4) + " maxima."
# TODO: add these to the ROI mgr
# TODO: as alternative, check out HMaxima transform from landini, basically subtracting the threshold before running the find maxima
def main():
rt = RT.open2(table_file.getAbsolutePath())
if not rt: return
log(" --- --- --- ")
log("Loaded %s" % table_file.getAbsolutePath())
log("Loading column lists...")
# Get column indices from imported file
headings = getColumnHeadings(rt)
id_col = getColumnIndex(headings, "TID")
t_col = getColumnIndex(headings, "t [")
d2p_col = getColumnIndex(headings, "D2P [")
angle_col = getColumnIndex(headings, u'\u03B1 [deg]')
delta_col = getColumnIndex(headings, u'\u0394\u03B1 [deg]')
if angle_col == RT.COLUMN_NOT_FOUND:
log("Failed to detect index for angle column. Re-trying...")
angle_col = getColumnIndex(headings, u'? [deg]')
if delta_col == RT.COLUMN_NOT_FOUND:
log("Failed to detect index for delta angle column. Re-trying...")
delta_col = getColumnIndex(headings, u'?? [deg]')
log("Last column index is %s" % rt.getLastColumn())
if RT.COLUMN_NOT_FOUND in (id_col, d2p_col, delta_col, angle_col):
uiservice.showDialog("Error: Some key columns were not found!", "Invalid Table?")
return
log("Settings: BOUT_WINDOW= %s, MIN_D2P= %s, DEF_FRAME_INTERVAL= %s"
% (BOUT_WINDOW, '{0:.4f}'.format(MIN_D2P), DEF_FRAME_INTERVAL))
# Store all data on dedicated lists
track_id_rows = rt.getColumnAsDoubles(id_col)
d2p_rows = rt.getColumnAsDoubles(d2p_col)
angle_rows = rt.getColumnAsDoubles(angle_col)
delta_rows = rt.getColumnAsDoubles(delta_col)
t_rows = rt.getColumnAsDoubles(t_col)
# Assess n of data points and extract unique path ids
n_rows = len(track_id_rows)
row_indices = range(n_rows)
track_ids = set(track_id_rows)
n_tracks = len(track_ids)
log("Table has %g rows" % n_rows)
log("Table has %g tracks" % n_tracks)
log("Parsing tracks...")
for track_id in track_ids:
for row, next_row in zip(row_indices, row_indices[1:]):
if track_id_rows[row] != track_id:
continue
if not isNumber(angle_rows[row]):
rt.setValue("FLAG", row, "NA")
continue
lower_bound = max(0, row - BOUT_WINDOW + 1)
upper_bound = min(n_rows-1, row + BOUT_WINDOW)
win_d2p = []
for _ in range(lower_bound, upper_bound):
win_d2p.append(d2p_rows[row])
if sum(win_d2p) <= MIN_D2P * len(win_d2p):
rt.setValue("FLAG", row, 0)
else:
current_angle = angle_rows[row]
next_angle = angle_rows[next_row]
current_delta = delta_rows[row]
flag = -1 if current_angle < 0 else 1
delta_change = (abs(current_delta) > 90)
same_sign = ((current_angle<0) == (next_angle<0))
if delta_change and not same_sign:
flag *= -1
rt.setValue("FLAG", row, flag)
if next_row == n_rows - 1:
rt.setValue("FLAG", next_row, flag)
if rt.save(table_file.getAbsolutePath()):
log("Processed table successfully saved (file overwritten)")
else:
log("Could not override input file. Displaying it...")
rt.show(table_file.name)
log("Creating onset table...")
onset_rt = RT()
onset_rt.showRowNumbers(False)
frame_int = DEF_FRAME_INTERVAL
if "table" in frame_rate_detection:
frame_int = getFrameIntervalFromTable(row_indices, track_id_rows, t_rows)
elif "image" in frame_rate_detection:
frame_int = getFrameIntervalFromImage(image_file.getAbsolutePath())
else:
log("Using default frame rate")
for track_id in track_ids:
for prev_row, row in zip(row_indices, row_indices[1:]):
if not track_id in (track_id_rows[prev_row], track_id_rows[row]):
continue
flag = rt.getValue("FLAG", row)
if not isNumber(flag):
continue
flag = int(flag)
if flag == 0:
continue
if flag == 1 or flag == -1:
srow = onset_rt.getCounter()
onset_rt.incrementCounter()
onset_rt.setValue("TID", srow, track_id)
from_frame = int(t_rows[prev_row]/frame_int) + 1
to_frame = int(t_rows[row]/frame_int) + 1
onset_rt.setValue("First disp. [t]", srow,
"%s to %s" % (t_rows[prev_row], t_rows[row]))
onset_rt.setValue("First disp. [frames]", srow,
"%s to %s" % (from_frame, to_frame))
onset_rt.setValue("ManualTag", srow, "")
break
out_path = suffixed_path(table_file.getAbsolutePath(), "ManualTagging")
if onset_rt.save(out_path):
log("Summary table successfully saved: %s" % out_path)
else:
log("File not saved... Displaying onset table")
onset_rt.show("Onsets %s" % table_file.name)