# Membrane, from the ovals: 312 points membrane = reduce( lambda cs, pol: [cs[0] + list(pol.xpoints), cs[1] + list(pol.ypoints)], [roi.getPolygon() for roi in rois], [[], []]) # Membrane oblique, fuzzy: another 76 points membrane_oblique = reduce( lambda cs, pol: [cs[0] + list(pol.xpoints), cs[1] + list(pol.ypoints)], [roi.getPolygon() for roi in [roi_oblique]], [[], []]) len_membrane = len(membrane[0]) + len(membrane_oblique[0]) # Background samples: as many as membrane samples rectangle = Roi(10, 10, w - 20, h - 20) pol = rectangle.getInterpolatedPolygon(1.0, False) # 433 points nonmem = (list(int(x) for x in pol.xpoints)[:len_membrane], list(int(y) for y in pol.ypoints)[:len_membrane]) #### import sys sys.path.append("/home/albert/lab/scripts/python/imagej/IsoView-GCaMP/") from lib.segmentation_em import createSMOClassifier, createRandomForestClassifier, classify, \ filterBank, filterBankRotations, filterBankBlockStatistics, \ createPerceptronClassifier, filterBankOrthogonalEdges, filterBankEdges from net.imglib2.img.display.imagej import ImageJFunctions as IL from net.imglib2.algorithm.math.ImgMath import offset from net.imglib2.view import Views from net.imglib2.type.numeric.real import DoubleType